Expression of protein phosphatase 1 during the asexual development of Neurospora crassa

T Zeke, E Kokai, B Szoor, E Yatzkan, O Yarden, K Szirak, Z Feher, P Bagossi, P Gergely, Z. Dombradi

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We cloned and sequenced the cDNA and the gene encoding the catalytic subunit of protein phosphatase I from the filamentous fungus Neurospora crassa. The gene, designated ppp-1 (phosphoprotein phosphatase 1), was mapped by restriction fragment length polymorphism to linkage group III, in the vicinity of con-7 and trp-1. The expression of the 0,gene was monitored by reverse transcriptase and polymerase chain reactions, by Western blotting, and by protein phosphatase activity assays in synchronized cultures. Transcripts of ppp-1 were detected in the dormant conidia. The abundance of ppp-1 mRNA, Ppp-1 protein, and the activity of protein phosphatase 1 increased during germination and subsequent hyphal elongation as well as during the early stages of aerial mycelium. formation. (C) 2002 Elsevier Science Inc. All rights reserved.

Original languageEnglish
Article numberPII S1096-4959(02)00188-4
Pages (from-to)161-170
Number of pages10
JournalComparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology
Volume134
Issue number1
Publication statusPublished - Jan 2003

Keywords / Materials (for Non-textual outputs)

  • developmental analysis
  • gene cloning
  • gene localization
  • phosphoprotein phosphatase 1
  • protein dephosphorylation
  • Neurospora crassa
  • CATALYTIC SUBUNIT
  • ASPERGILLUS-NIDULANS
  • COMPLETION
  • ENCODES
  • GENES
  • 2A

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