Expression of putative markers of pluripotency in equine embryonic and adult tissues

Cristina L Esteves; Ruchi Sharma; Lucy Dawson; Sarah E Taylor; Gemma Pearson; John A Keen; Kieran McDonald; Christine Aurich; F Xavier Donadeu

doi:10.1016/j.tvjl.2014.08.026

Expression of putative markers of pluripotency in equine embryonic and adult tissues

Cristina L Esteves, Ruchi Sharma, Lucy Dawson, Sarah E Taylor, Gemma Pearson, John A Keen, Kieran McDonald, Christine Aurich, F Xavier Donadeu

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Expression of several putative markers of pluripotency (OCT4, SOX2, NANOG, LIN28A, REX1, DNMT3B and TERT) was examined in a range of equine tissues, including early embryos, induced pluripotent stem cells (iPSCs), testis, adipose- and bone marrow-derived mesenchymal stromal cells (MSCs), and keratinocytes. Transcript levels of all markers were highest in embryos and iPSCs and, except for SOX2, were very low or undetectable in keratinocytes. Mean expression levels of all markers were lower in testis than in embryos or iPSCs and, except for DNMT3B, were higher in testis than in MSCs. Expression of OCT4, NANOG and DNMT3B, but not the other markers, was detected in MSCs. Of all markers analysed, only LIN28A, REX1 and TERT were associated exclusively with pluripotent cells in the horse.

Original language	English
Pages (from-to)	533-535
Journal	Veterinary Journal
Volume	202
Issue number	3
Early online date	29 Aug 2014
DOIs	https://doi.org/10.1016/j.tvjl.2014.08.026
Publication status	Published - Dec 2014

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10.1016/j.tvjl.2014.08.026

http://www.sciencedirect.com/science/article/pii/S1090023314003633

Derivation of pluripotent stem cells from equine fibroblasts
Donadeu, X., Burdon, T. & Whitelaw, B.
UK central government bodies/local authorities, health and hospital authorities
1/02/09 → 31/01/12
Project: Research

Sarah Taylor
- sarah.e.taylored.acuk
- Royal (Dick) School of Veterinary Studies - Senior Lecturer in Equine Orthopaedics
Person: Academic: Research Active

Cite this

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title = "Expression of putative markers of pluripotency in equine embryonic and adult tissues",

abstract = "Expression of several putative markers of pluripotency (OCT4, SOX2, NANOG, LIN28A, REX1, DNMT3B and TERT) was examined in a range of equine tissues, including early embryos, induced pluripotent stem cells (iPSCs), testis, adipose- and bone marrow-derived mesenchymal stromal cells (MSCs), and keratinocytes. Transcript levels of all markers were highest in embryos and iPSCs and, except for SOX2, were very low or undetectable in keratinocytes. Mean expression levels of all markers were lower in testis than in embryos or iPSCs and, except for DNMT3B, were higher in testis than in MSCs. Expression of OCT4, NANOG and DNMT3B, but not the other markers, was detected in MSCs. Of all markers analysed, only LIN28A, REX1 and TERT were associated exclusively with pluripotent cells in the horse.",

author = "Esteves, {Cristina L} and Ruchi Sharma and Lucy Dawson and Taylor, {Sarah E} and Gemma Pearson and Keen, {John A} and Kieran McDonald and Christine Aurich and Donadeu, {F Xavier}",

year = "2014",

month = dec,

doi = "10.1016/j.tvjl.2014.08.026",

language = "English",

volume = "202",

pages = "533--535",

journal = "Veterinary Journal",

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T1 - Expression of putative markers of pluripotency in equine embryonic and adult tissues

AU - Esteves, Cristina L

AU - Sharma, Ruchi

AU - Dawson, Lucy

AU - Taylor, Sarah E

AU - Pearson, Gemma

AU - Keen, John A

AU - McDonald, Kieran

AU - Aurich, Christine

AU - Donadeu, F Xavier

PY - 2014/12

Y1 - 2014/12

N2 - Expression of several putative markers of pluripotency (OCT4, SOX2, NANOG, LIN28A, REX1, DNMT3B and TERT) was examined in a range of equine tissues, including early embryos, induced pluripotent stem cells (iPSCs), testis, adipose- and bone marrow-derived mesenchymal stromal cells (MSCs), and keratinocytes. Transcript levels of all markers were highest in embryos and iPSCs and, except for SOX2, were very low or undetectable in keratinocytes. Mean expression levels of all markers were lower in testis than in embryos or iPSCs and, except for DNMT3B, were higher in testis than in MSCs. Expression of OCT4, NANOG and DNMT3B, but not the other markers, was detected in MSCs. Of all markers analysed, only LIN28A, REX1 and TERT were associated exclusively with pluripotent cells in the horse.

AB - Expression of several putative markers of pluripotency (OCT4, SOX2, NANOG, LIN28A, REX1, DNMT3B and TERT) was examined in a range of equine tissues, including early embryos, induced pluripotent stem cells (iPSCs), testis, adipose- and bone marrow-derived mesenchymal stromal cells (MSCs), and keratinocytes. Transcript levels of all markers were highest in embryos and iPSCs and, except for SOX2, were very low or undetectable in keratinocytes. Mean expression levels of all markers were lower in testis than in embryos or iPSCs and, except for DNMT3B, were higher in testis than in MSCs. Expression of OCT4, NANOG and DNMT3B, but not the other markers, was detected in MSCs. Of all markers analysed, only LIN28A, REX1 and TERT were associated exclusively with pluripotent cells in the horse.

U2 - 10.1016/j.tvjl.2014.08.026

DO - 10.1016/j.tvjl.2014.08.026

M3 - Article

C2 - 25241949

SN - 1090-0233

VL - 202

SP - 533

EP - 535

JO - Veterinary Journal

JF - Veterinary Journal

IS - 3

ER -

Expression of putative markers of pluripotency in equine embryonic and adult tissues

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Projects

Derivation of pluripotent stem cells from equine fibroblasts

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Sarah Taylor

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