Expression of putative markers of pluripotency in equine embryonic and adult tissues

Cristina L Esteves, Ruchi Sharma, Lucy Dawson, Sarah E Taylor, Gemma Pearson, John A Keen, Kieran McDonald, Christine Aurich, F Xavier Donadeu

Research output: Contribution to journalArticlepeer-review


Expression of several putative markers of pluripotency (OCT4, SOX2, NANOG, LIN28A, REX1, DNMT3B and TERT) was examined in a range of equine tissues, including early embryos, induced pluripotent stem cells (iPSCs), testis, adipose- and bone marrow-derived mesenchymal stromal cells (MSCs), and keratinocytes. Transcript levels of all markers were highest in embryos and iPSCs and, except for SOX2, were very low or undetectable in keratinocytes. Mean expression levels of all markers were lower in testis than in embryos or iPSCs and, except for DNMT3B, were higher in testis than in MSCs. Expression of OCT4, NANOG and DNMT3B, but not the other markers, was detected in MSCs. Of all markers analysed, only LIN28A, REX1 and TERT were associated exclusively with pluripotent cells in the horse.

Original languageEnglish
Pages (from-to)533-535
JournalVeterinary Journal
Issue number3
Early online date29 Aug 2014
Publication statusPublished - Dec 2014


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