Expression of the prion-like protein Shadoo in the developing mouse embryo

Rachel Young, Stéphan Bouet, Jacqueline Polyte, Sandrine Le Guillou, Bruno Passet, Marthe Vilotte, Johan Castille, Vincent Beringue, Fabienne Le Provost, Hubert Laude, Jean-Luc Vilotte

Research output: Contribution to journalArticlepeer-review

Abstract

The prion-like protein Shadoo has been suggested to compensate for the lack of PrP in Prnp-knockout mice, explaining their lack of extreme phenotype. In adult mice, both PrP and Shadoo have shown overlapping expression patterns and shared functions. Their expression in the mouse embryo has also been suggested to be complementary, as invalidation of both genes results in embryonic lethality. The developmental expression profile of PrP has been described from post-implantation stages up until birth. However the spatial expression pattern of Shadoo in the developing mouse embryo is not known. We previously described the expression profile of the prion-like protein Shadoo in adult mice using Sprn reporter mice (Sprn-GFP and Sprn-LacZ). Here we used these mice to describe the developmental expression of Shadoo between 10.5 and 14.5 dpc. The observed pattern in specific embryonic cell lineages and in extra-embryonic tissues is consistent with the previously reported phenotype resulting from its knockdown.

Original languageEnglish
Pages (from-to)184-7
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume416
Issue number1-2
DOIs
Publication statusPublished - 9 Dec 2011
Externally publishedYes

Keywords

  • Animals
  • Cell Lineage
  • Embryo, Mammalian
  • Gene Knockdown Techniques
  • Mice
  • Mice, Transgenic
  • Nerve Tissue Proteins
  • Prion Proteins
  • Prions
  • Transgenes
  • beta-Galactosidase

Fingerprint

Dive into the research topics of 'Expression of the prion-like protein Shadoo in the developing mouse embryo'. Together they form a unique fingerprint.

Cite this