Expression profiling reveals novel innate and inflammatory responses in the jejunal epithelial compartment during infection with Trichinella spiralis

Pamela A Knight, Alan D Pemberton, Kevin A Robertson, Douglas J Roy, Steven H Wright, Hugh R P Miller

Research output: Contribution to journalArticlepeer-review

Abstract

Infection with intestinal nematodes induces profound pathological changes to the gut that are associated with eventual parasite expulsion. We have applied expression profiling as an initial screening process with oligonucleotide microarrays (Affymetrix MG-U74AV2 gene chips) and time course kinetics to investigate gene transcription triggered by the intraepithelial nematode Trichinella spiralis in jejunal epithelium from BALB/c mice. Of the 4,114 genes detected, 2,617 were present in all uninfected and T. spiralis-infected replicates, 8% of which were notably upregulated, whereas 12% were downregulated at the time of worm expulsion (day 14 postinfection). Upregulation of goblet cell mucin gene transcripts intestinal mucin gene 3 (MUC3), calcium chloride channel 5 (CLCA5), and goblet cell gene 4 (GOB4) is consistent with enhanced production and alteration of mucus, whereas a 60- to 70-fold upregulation of transcripts for mast cell proteases 1 and 2 (MCPT-1 and -2) is consistent with intraepithelial mucosal mast cell recruitment. Importantly, there was novel expression of sialyltransferase 4C (SIAT4C), small proline-rich protein 2A (SPRR2A), and resistin-like molecule beta (RELMbeta) on day 14 postinfection. In contrast, DNase I and regenerating protein 3 (REG3) transcripts were substantially downregulated. Time course analyses revealed early (within 48 h of infection) induction of Siat4c, Sprr2A, and Relmbeta and later (within 120 h) induction of Mcpt-1 and -2. The findings demonstrate early innate responses and later inflammatory changes within the epithelium. The early epithelial responses may be associated both with repair (Sprr2A) and with the development of innate immunity (Siat4c and Relmbeta).
Original languageEnglish
Pages (from-to)6076-86
Number of pages11
JournalInfection and Immunity
Volume72
Issue number10
DOIs
Publication statusPublished - 2004

Keywords

  • Animals
  • Antioxidants
  • Cytoskeleton
  • Epithelial Cells
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Glutathione
  • Goblet Cells
  • Immunity
  • Inflammation
  • Jejunum
  • Male
  • Mast Cells
  • Membrane Proteins
  • Mice
  • Mice, Inbred BALB C
  • Mucins
  • Oligonucleotide Array Sequence Analysis
  • Organ Specificity
  • Paneth Cells
  • Tight Junctions
  • Transcription, Genetic
  • Trichinella spiralis
  • Trichinellosis

Fingerprint

Dive into the research topics of 'Expression profiling reveals novel innate and inflammatory responses in the jejunal epithelial compartment during infection with Trichinella spiralis'. Together they form a unique fingerprint.

Cite this