Expression, purification and preliminary crystallographic studies of a single-point mutant of Mos1 mariner transposase

J M  Richardson; Lei Zhang; S  Marcos; D J  Finnegan; M M  Harding; P  Taylor; M D  Walkinshaw

doi:10.1107/S0907444904003798

Expression, purification and preliminary crystallographic studies of a single-point mutant of Mos1 mariner transposase

J M Richardson, Lei Zhang, S Marcos, D J Finnegan, M M Harding, P Taylor, M D Walkinshaw

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A soluble single-point mutant of full-length Mos1 mariner transposase (MW = 40.7 kDa) has been overexpressed in Escherichia coli, purified to 95% homogeneity and crystallized. This provides the first example of the crystallization of a eukaryotic transposase. The native crystals diffract to 2.5 Angstrom resolution and show tetragonal symmetry, with unit-cell parameters a = b = 44.5, c = 205.6 Angstrom. Multiple-wavelength anomalous data from a selenomethionyl form of the protein and data from a heavy-atom derivative have been collected.

Original language	English
Pages (from-to)	962-964
Number of pages	3
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	60
Issue number	Pt 5
DOIs	https://doi.org/10.1107/S0907444904003798
Publication status	Published - May 2004

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Targeted integration and the mechanism of transposition of a mariner transposable element
Finnegan, D. (Principal Investigator)
Biotechnology and Biological Sciences Research Council
1/09/04 → 31/10/07
Project: Research

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abstract = "A soluble single-point mutant of full-length Mos1 mariner transposase (MW = 40.7 kDa) has been overexpressed in Escherichia coli, purified to 95\% homogeneity and crystallized. This provides the first example of the crystallization of a eukaryotic transposase. The native crystals diffract to 2.5 Angstrom resolution and show tetragonal symmetry, with unit-cell parameters a = b = 44.5, c = 205.6 Angstrom. Multiple-wavelength anomalous data from a selenomethionyl form of the protein and data from a heavy-atom derivative have been collected.",

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AU - Zhang, Lei

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AU - Harding, M M

AU - Taylor, P

AU - Walkinshaw, M D

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Expression, purification and preliminary crystallographic studies of a single-point mutant of Mos1 mariner transposase

Abstract

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Targeted integration and the mechanism of transposition of a mariner transposable element

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