Extensive Hematopoietic Stem Cell Generation in the AGM Region via Maturation of VE-Cadherin+CD45+ Pre-Definitive HSCs

Samir Taoudi, Christèle Gonneau, Kate Moore, Julie M Sheridan, C Clare Blackburn, Erin Taylor, Alexander Medvinsky

Research output: Contribution to journalArticlepeer-review

Abstract

Elucidating the mechanisms underlying hematopoietic stem cell (HSC) specification and expansion in the embryo has been hampered by the lack of analytical cell culture systems that recapitulate in vivo development. Here, we describe an ex vivo model that facilitates a rapid and robust emergence of multipotent long-term repopulating HSCs in the embryonic AGM region. Because this method includes a cell dissociation step prior to reconstruction of a three-dimensional functional tissue and preserves both stromal and hematopoietic elements, it allowed us to identify the direct ancestry of the rapidly expanding HSC pool. We demonstrate that extensive generation of definitive HSCs in the AGM occurs predominantly through the acquisition of stem characteristics by the VE-cadherin+CD45+ population.
Original languageEnglish
Pages (from-to)99-108
Number of pages10
JournalCell Stem Cell
Volume3
Issue number1
DOIs
Publication statusPublished - Jul 2008

Keywords

  • STEMCELL

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