Extra-ovarian production of mature viable mouse oocytes from frozen primary follicles

J. Carroll; D. G. Whittingham; M. J.  Wood; E. Telfer; R. G. Gosden

doi:10.1530/jrf.0.0900321

Extra-ovarian production of mature viable mouse oocytes from frozen primary follicles

J. Carroll, D. G. Whittingham^*, M. J. Wood, E. Telfer, R. G. Gosden

^*Corresponding author for this work

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Isolated primary mouse follicles can be frozen successfully and thawed in the presence of 1.5 M-DMSO. Similar proportions of freshly collected and frozen-thawed primary follicles undergo folliculogenesis in the absence of other ovarian tissue. Some of the mature oocytes recovered from these follicles were fertilized in vitro and, after transfer to pseudopregnant recipients at the 2-cell stage, developed into live young. Cryopreservation and extra-ovarian development of immature follicles provide a unique opportunity to store large numbers of female gametes.

Original language	English
Pages (from-to)	321-327
Number of pages	7
Journal	Journal of reproduction and fertility
Volume	90
Issue number	1
DOIs	https://doi.org/10.1530/jrf.0.0900321
Publication status	Published - Sept 1990

Keywords / Materials (for Non-textual outputs)

Cryopreservation
Mouse
Oocyte
Primary follicle
In-vitro folliculogenesis
Oocyte maturation
In-vitro fertilization
Embryo transfer

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10.1530/jrf.0.0900321

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title = "Extra-ovarian production of mature viable mouse oocytes from frozen primary follicles",

abstract = "Isolated primary mouse follicles can be frozen successfully and thawed in the presence of 1.5 M-DMSO. Similar proportions of freshly collected and frozen-thawed primary follicles undergo folliculogenesis in the absence of other ovarian tissue. Some of the mature oocytes recovered from these follicles were fertilized in vitro and, after transfer to pseudopregnant recipients at the 2-cell stage, developed into live young. Cryopreservation and extra-ovarian development of immature follicles provide a unique opportunity to store large numbers of female gametes.",

keywords = "Cryopreservation, Mouse, Oocyte, Primary follicle, In-vitro folliculogenesis, Oocyte maturation, In-vitro fertilization, Embryo transfer",

author = "J. Carroll and Whittingham, {D. G.} and Wood, {M. J.} and E. Telfer and Gosden, {R. G.}",

note = "We thank Professor C. D. Matthews for provision of a Reproductive Medicine Postgraduate Scholarship to J.C.",

year = "1990",

month = sep,

doi = "10.1530/jrf.0.0900321",

language = "English",

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T1 - Extra-ovarian production of mature viable mouse oocytes from frozen primary follicles

AU - Carroll, J.

AU - Whittingham, D. G.

AU - Wood, M. J.

AU - Telfer, E.

AU - Gosden, R. G.

N1 - We thank Professor C. D. Matthews for provision of a Reproductive Medicine Postgraduate Scholarship to J.C.

PY - 1990/9

Y1 - 1990/9

N2 - Isolated primary mouse follicles can be frozen successfully and thawed in the presence of 1.5 M-DMSO. Similar proportions of freshly collected and frozen-thawed primary follicles undergo folliculogenesis in the absence of other ovarian tissue. Some of the mature oocytes recovered from these follicles were fertilized in vitro and, after transfer to pseudopregnant recipients at the 2-cell stage, developed into live young. Cryopreservation and extra-ovarian development of immature follicles provide a unique opportunity to store large numbers of female gametes.

AB - Isolated primary mouse follicles can be frozen successfully and thawed in the presence of 1.5 M-DMSO. Similar proportions of freshly collected and frozen-thawed primary follicles undergo folliculogenesis in the absence of other ovarian tissue. Some of the mature oocytes recovered from these follicles were fertilized in vitro and, after transfer to pseudopregnant recipients at the 2-cell stage, developed into live young. Cryopreservation and extra-ovarian development of immature follicles provide a unique opportunity to store large numbers of female gametes.

KW - Cryopreservation

KW - Mouse

KW - Oocyte

KW - Primary follicle

KW - In-vitro folliculogenesis

KW - Oocyte maturation

KW - In-vitro fertilization

KW - Embryo transfer

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DO - 10.1530/jrf.0.0900321

M3 - Article

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AN - SCOPUS:0025091519

SN - 0022-4251

VL - 90

SP - 321

EP - 327

JO - Journal of reproduction and fertility

JF - Journal of reproduction and fertility

IS - 1

ER -

Extra-ovarian production of mature viable mouse oocytes from frozen primary follicles

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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