Extracellular cross-linking of maize arabinoxylans by oxidation of feruloyl esters to form oligoferuloyl esters and ether-like bonds

Sally J. Burr, Stephen C. Fry

Research output: Contribution to journalArticlepeer-review

Abstract

Primary cell walls of grasses and cereals contain arabinoxylans with esterified ferulate side chains, which are proposed to cross-link the polysaccharides during maturation by undergoing oxidative coupling. However, the mechanisms and control of arabinoxylan cross-linking in vivo are unclear. Non-lignifying maize (Zea mays L.) cell cultures were incubated with l-[1-H-3]arabinose or (E)-[U-C-14]cinnamate (radiolabelling the pentosyl and feruloyl groups of endogenous arabinoxylans, respectively), or with exogenous feruloyl-[H-3]arabinoxylans. The cross-linking rate of soluble extracellular arabinoxylans, monitored on Sepharose CL-2B, peaked suddenly and transiently, typically at similar to 9 days after subculture. This peak was not associated with appreciable changes in peroxidase activity, and was probably governed by fluctuations in H2O2 and/or inhibitors. De-esterified arabinoxylans failed to cross-link, supporting a role for the feruloyl ester groups. The cross-links were stable in vivo. Some of them also withstood mild alkaline conditions, indicating that they were not (only) based on ester bonds; however, most were cleaved by 6 m NaOH, which is a property of p-hydroxybenzyl-sugar ether bonds. Cross-linking of [C-14]feruloyl-arabinoxylans also occurred in vitro, in the presence of endogenous peroxidases plus exogenous H2O2. During cross-linking, the feruloyl groups were oxidized, as shown by ultraviolet spectra and thin-layer chromatography. Esterified diferulates were minor oxidation products; major products were: (i) esterified oligoferulates, released by treatment with mild alkali; and (ii) phenolic components attached to polysaccharides via relatively alkali-stable (ether-like) bonds. Thus, feruloyl esters participate in polysaccharide cross-linking, but mainly by oligomerization rather than by dimerization. We propose that, after the oxidative coupling, strong p-hydroxybenzyl-polysaccharide ether bonds are formed via quinone-methide intermediates.

Original languageEnglish
Pages (from-to)554-567
Number of pages14
JournalThe Plant Journal
Volume58
Issue number4
Early online date26 Feb 2009
DOIs
Publication statusPublished - 31 May 2009

Keywords

  • Poaceae
  • primary cell walls
  • ferulic cid
  • arabinoxylan
  • oxidative coupling
  • hemicellulose

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