Extracellular Vesicles-Mediated Bio-Orthogonal Catalysis in Growing Tumors

Maria Sancho-Albero, Victor Sebastian, Ana Perez-Lopez, Pilar Martin-Duque, Asier Unciti-Broceta, Jesús Santamaría

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Several studies have reported the successful use of bio-orthogonal catalyst nanoparticles (NPs) for cancer therapy. However, the delivery of the catalysts to the target tissues in vivo remains an unsolved challenge. The combination of catalytic NPs with extracellular vesicles (EVs) has been proposed as a promising approach to improve the delivery of therapeutic nanomaterials to the desired organs. In this study, we have developed a nanoscale bio-hybrid vector using a CO-mediated reduction at low temperature to generate ultrathin catalytic Pd nanosheets (PdNSs) as catalysts directly inside cancer-derived EVs. We have also compared their biodistribution with that of PEGylated PdNSs delivered by the EPR effect. Our results indicate that the accumulation of PdNSs in the tumour tissue was significantly higher when they were administered within the EVs compared to the PEGylated PdNSs. Conversely, the amount of Pd found in non-target organs (i.e., liver) was lowered. Once the Pd-based catalytic EVs were accumulated in the tumours, they ena-bled the activation of a paclitaxel prodrug demonstrating their ability to carry out bio-orthogonal uncaging chemistries in vivo for cancer therapy
Original languageEnglish
JournalCells
Early online date16 Apr 2024
DOIs
Publication statusE-pub ahead of print - 16 Apr 2024

Keywords / Materials (for Non-textual outputs)

  • extracellular vesicles
  • bio-orthogonal catalysis
  • cancer
  • palladium nanosheets

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