Extraction and LC-MS/MS analysis of four steroids from mouse plasma and bone marrow

William P Cawthorn; Natalie Z M Homer; Scott G. Denham; Andrea Lovdel

doi:10.17504/protocols.io.e6nvwdrmzlmk/v1

Extraction and LC-MS/MS analysis of four steroids from mouse plasma and bone marrow

William P Cawthorn, Natalie Z M Homer^*, Scott G. Denham, Andrea Lovdel

^*Corresponding author for this work

Research output: Other contribution › Protocols

Original language	English
Type	LC-MS/MS method
Publisher	protocols.io
DOIs	https://doi.org/10.17504/protocols.io.e6nvwdrmzlmk/v1
Publication status	Published - 20 Feb 2024

Keywords / Materials (for Non-textual outputs)

steroid
steroid analysis
quantitative steroid measurement
LC-MS/MS
corticosterone
11-dehydrocorticosterone
testosterone
progesterone
supported liquid extraction
mouse plasma
mouse bone marrow
bone marrow
mouse
tissue specific
tandem mass spectrometry
steroid mass spectrometry

Access to Document

10.17504/protocols.io.e6nvwdrmzlmk/v1Licence: Creative Commons: Attribution (CC-BY)

http://dx.doi.org/10.17504/protocols.io.e6nvwdrmzlmk/v1Licence: Creative Commons: Attribution (CC-BY)

Bone marrow adipose tissue as a novel regulator of metabolic homeostasis
Cawthorn, W.
MRC
1/08/15 → 30/06/22
Project: Research

Edinburgh Clinical Research Facility Mass Spectrometry Core in the QMRI
Natalie Homer (Manager), Scott Denham (Other) & Jo Simpson (Other)
Centre for Cardiovascular Science
Facility/equipment: Facility

Cite this

@misc{ed42a80ccf9c420584c0ea7f80d67ea9,

title = "Extraction and LC-MS/MS analysis of four steroids from mouse plasma and bone marrow",

keywords = "steroid, steroid analysis, quantitative steroid measurement, LC-MS/MS, corticosterone, 11-dehydrocorticosterone, testosterone, progesterone, supported liquid extraction, mouse plasma, mouse bone marrow, bone marrow, mouse, tissue specific, tandem mass spectrometry, steroid mass spectrometry",

author = "Cawthorn, {William P} and Homer, {Natalie Z M} and Denham, {Scott G.} and Andrea Lovdel",

year = "2024",

month = feb,

day = "20",

doi = "10.17504/protocols.io.e6nvwdrmzlmk/v1",

language = "English",

publisher = "protocols.io",

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T1 - Extraction and LC-MS/MS analysis of four steroids from mouse plasma and bone marrow

AU - Cawthorn, William P

AU - Homer, Natalie Z M

AU - Denham, Scott G.

AU - Lovdel, Andrea

PY - 2024/2/20

Y1 - 2024/2/20

KW - steroid

KW - steroid analysis

KW - quantitative steroid measurement

KW - LC-MS/MS

KW - corticosterone

KW - 11-dehydrocorticosterone

KW - testosterone

KW - progesterone

KW - supported liquid extraction

KW - mouse plasma

KW - mouse bone marrow

KW - bone marrow

KW - mouse

KW - tissue specific

KW - tandem mass spectrometry

KW - steroid mass spectrometry

U2 - 10.17504/protocols.io.e6nvwdrmzlmk/v1

DO - 10.17504/protocols.io.e6nvwdrmzlmk/v1

M3 - Protocols

PB - protocols.io

ER -

Extraction and LC-MS/MS analysis of four steroids from mouse plasma and bone marrow

Keywords / Materials (for Non-textual outputs)

Access to Document

Projects

Bone marrow adipose tissue as a novel regulator of metabolic homeostasis

Equipment

Edinburgh Clinical Research Facility Mass Spectrometry Core in the QMRI

Cite this