The extraction of xyloglucan from Rosa suspension-culture cell walls (not depectinated in hot aqueous solutions) with alkali was dependent on the temperature, with 2-3 times more being extracted at 37° than at 25°. At 25°, 6m NaOH was the optimal concentration, but it extracted only 25-30% of the xyloglucan within 24 h; efficient extraction took 2-3 weeks. After pre-treatment of cell walls with 6m NaOH at 25°, water extracted more xyloglucan in 24 h than did additional 6m NaOH.
|Number of pages||9|
|Publication status||Published - 27 Apr 1992|