Factors that affect the extraction of xyloglucan from the primary cell walls of suspension-cultured rose cells

Stephen C. Fry

doi:10.1016/0008-6215(92)84135-F

Factors that affect the extraction of xyloglucan from the primary cell walls of suspension-cultured rose cells

Stephen C. Fry^*

^*Corresponding author for this work

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The extraction of xyloglucan from Rosa suspension-culture cell walls (not depectinated in hot aqueous solutions) with alkali was dependent on the temperature, with 2-3 times more being extracted at 37° than at 25°. At 25°, 6m NaOH was the optimal concentration, but it extracted only 25-30% of the xyloglucan within 24 h; efficient extraction took 2-3 weeks. After pre-treatment of cell walls with 6m NaOH at 25°, water extracted more xyloglucan in 24 h than did additional 6m NaOH.

Original language	English
Pages (from-to)	423-431
Number of pages	9
Journal	Carbohydrate Research
Volume	228
Issue number	2
DOIs	https://doi.org/10.1016/0008-6215(92)84135-F
Publication status	Published - 27 Apr 1992

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title = "Factors that affect the extraction of xyloglucan from the primary cell walls of suspension-cultured rose cells",

abstract = "The extraction of xyloglucan from Rosa suspension-culture cell walls (not depectinated in hot aqueous solutions) with alkali was dependent on the temperature, with 2-3 times more being extracted at 37° than at 25°. At 25°, 6m NaOH was the optimal concentration, but it extracted only 25-30% of the xyloglucan within 24 h; efficient extraction took 2-3 weeks. After pre-treatment of cell walls with 6m NaOH at 25°, water extracted more xyloglucan in 24 h than did additional 6m NaOH.",

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AB - The extraction of xyloglucan from Rosa suspension-culture cell walls (not depectinated in hot aqueous solutions) with alkali was dependent on the temperature, with 2-3 times more being extracted at 37° than at 25°. At 25°, 6m NaOH was the optimal concentration, but it extracted only 25-30% of the xyloglucan within 24 h; efficient extraction took 2-3 weeks. After pre-treatment of cell walls with 6m NaOH at 25°, water extracted more xyloglucan in 24 h than did additional 6m NaOH.

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Factors that affect the extraction of xyloglucan from the primary cell walls of suspension-cultured rose cells

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