Factors that affect the extraction of xyloglucan from the primary cell walls of suspension-cultured rose cells

Stephen C. Fry*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The extraction of xyloglucan from Rosa suspension-culture cell walls (not depectinated in hot aqueous solutions) with alkali was dependent on the temperature, with 2-3 times more being extracted at 37° than at 25°. At 25°, 6m NaOH was the optimal concentration, but it extracted only 25-30% of the xyloglucan within 24 h; efficient extraction took 2-3 weeks. After pre-treatment of cell walls with 6m NaOH at 25°, water extracted more xyloglucan in 24 h than did additional 6m NaOH.

Original languageEnglish
Pages (from-to)423-431
Number of pages9
JournalCarbohydrate Research
Volume228
Issue number2
DOIs
Publication statusPublished - 27 Apr 1992

Fingerprint

Dive into the research topics of 'Factors that affect the extraction of xyloglucan from the primary cell walls of suspension-cultured rose cells'. Together they form a unique fingerprint.

Cite this