Failure to detect Plasmodium vivax in West and Central Africa by PCR species typing

Richard L. Culleton; Toshihiro Mita; Mathieu Ndounga; Holger Unger; Pedro V. L. Cravo; Giacomo M. Paganotti; Nobuyuki Takahashi; Akira Kaneko; Hideaki Eto; Halidou Tinto; Corine Karema; Umberto D'Alessandro; Virgilio do Rosario; Takatoshi Kobayakawa; Francine Ntoumi; Richard Carter; Kazuyuki Tanabe

doi:10.1186/1475-2875-7-174

Failure to detect Plasmodium vivax in West and Central Africa by PCR species typing

Richard L. Culleton, Toshihiro Mita, Mathieu Ndounga, Holger Unger, Pedro V. L. Cravo, Giacomo M. Paganotti, Nobuyuki Takahashi, Akira Kaneko, Hideaki Eto, Halidou Tinto, Corine Karema, Umberto D'Alessandro, Virgilio do Rosario, Takatoshi Kobayakawa, Francine Ntoumi, Richard Carter, Kazuyuki Tanabe

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Plasmodium vivax is estimated to affect 75 million people annually. It is reportedly absent, however, from west and central Africa due to the high prevalence of the Duffy negative phenotype in the indigenous populations. Despite this, non-African travellers consistently return to their own countries with P. vivax malaria after visiting this region. An attempt was made, therefore, to detect the presence of P. vivax parasites in blood samples collected from the indigenous populations of west and central Africa.

Methods: Parasite species typing (for all four human malaria parasites) was carried out by PCR on 2,588 blood samples collected from individuals from nine African malaria-endemic countries.

Results: Most infections (98.5%) were Plasmodium falciparum, Plasmodium malariae was identified in 8.5% of all infections, and Plasmodium ovale in 3.9%. The prevalence of both parasites varied greatly by country. Only one case of P. vivax was detected from Sao Tome, an island off the west coast of Africa, confirming the scarcity of this parasite in Africa.

Conclusion: The prevalence of P. vivax in local populations in sub-Saharan Africa is very low, despite the frequent identification of this parasite in non-African travellers.

Original language	English
Article number	174
Pages (from-to)	-
Number of pages	8
Journal	Malaria Journal
Volume	7
DOIs	https://doi.org/10.1186/1475-2875-7-174
Publication status	Published - 11 Sep 2008

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Culleton, R. L., Mita, T., Ndounga, M., Unger, H., Cravo, P. V. L., Paganotti, G. M., Takahashi, N., Kaneko, A., Eto, H., Tinto, H., Karema, C., D'Alessandro, U., do Rosario, V., Kobayakawa, T., Ntoumi, F., Carter, R., & Tanabe, K. (2008). Failure to detect Plasmodium vivax in West and Central Africa by PCR species typing. Malaria Journal, 7, -. [174]. https://doi.org/10.1186/1475-2875-7-174

Culleton, Richard L. ; Mita, Toshihiro ; Ndounga, Mathieu ; Unger, Holger ; Cravo, Pedro V. L. ; Paganotti, Giacomo M. ; Takahashi, Nobuyuki ; Kaneko, Akira ; Eto, Hideaki ; Tinto, Halidou ; Karema, Corine ; D'Alessandro, Umberto ; do Rosario, Virgilio ; Kobayakawa, Takatoshi ; Ntoumi, Francine ; Carter, Richard ; Tanabe, Kazuyuki. / Failure to detect Plasmodium vivax in West and Central Africa by PCR species typing. In: Malaria Journal. 2008 ; Vol. 7. pp. -.

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title = "Failure to detect Plasmodium vivax in West and Central Africa by PCR species typing",

abstract = "Background: Plasmodium vivax is estimated to affect 75 million people annually. It is reportedly absent, however, from west and central Africa due to the high prevalence of the Duffy negative phenotype in the indigenous populations. Despite this, non-African travellers consistently return to their own countries with P. vivax malaria after visiting this region. An attempt was made, therefore, to detect the presence of P. vivax parasites in blood samples collected from the indigenous populations of west and central Africa.Methods: Parasite species typing (for all four human malaria parasites) was carried out by PCR on 2,588 blood samples collected from individuals from nine African malaria-endemic countries.Results: Most infections (98.5%) were Plasmodium falciparum, Plasmodium malariae was identified in 8.5% of all infections, and Plasmodium ovale in 3.9%. The prevalence of both parasites varied greatly by country. Only one case of P. vivax was detected from Sao Tome, an island off the west coast of Africa, confirming the scarcity of this parasite in Africa.Conclusion: The prevalence of P. vivax in local populations in sub-Saharan Africa is very low, despite the frequent identification of this parasite in non-African travellers.",

author = "Culleton, {Richard L.} and Toshihiro Mita and Mathieu Ndounga and Holger Unger and Cravo, {Pedro V. L.} and Paganotti, {Giacomo M.} and Nobuyuki Takahashi and Akira Kaneko and Hideaki Eto and Halidou Tinto and Corine Karema and Umberto D'Alessandro and {do Rosario}, Virgilio and Takatoshi Kobayakawa and Francine Ntoumi and Richard Carter and Kazuyuki Tanabe",

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doi = "10.1186/1475-2875-7-174",

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Culleton, RL, Mita, T, Ndounga, M, Unger, H, Cravo, PVL, Paganotti, GM, Takahashi, N, Kaneko, A, Eto, H, Tinto, H, Karema, C, D'Alessandro, U, do Rosario, V, Kobayakawa, T, Ntoumi, F, Carter, R & Tanabe, K 2008, 'Failure to detect Plasmodium vivax in West and Central Africa by PCR species typing', Malaria Journal, vol. 7, 174, pp. -. https://doi.org/10.1186/1475-2875-7-174

Failure to detect Plasmodium vivax in West and Central Africa by PCR species typing. / Culleton, Richard L.; Mita, Toshihiro; Ndounga, Mathieu; Unger, Holger; Cravo, Pedro V. L.; Paganotti, Giacomo M.; Takahashi, Nobuyuki; Kaneko, Akira; Eto, Hideaki; Tinto, Halidou; Karema, Corine; D'Alessandro, Umberto; do Rosario, Virgilio; Kobayakawa, Takatoshi; Ntoumi, Francine; Carter, Richard; Tanabe, Kazuyuki.

In: Malaria Journal, Vol. 7, 174, 11.09.2008, p. -.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Failure to detect Plasmodium vivax in West and Central Africa by PCR species typing

AU - Culleton, Richard L.

AU - Mita, Toshihiro

AU - Ndounga, Mathieu

AU - Unger, Holger

AU - Cravo, Pedro V. L.

AU - Paganotti, Giacomo M.

AU - Takahashi, Nobuyuki

AU - Kaneko, Akira

AU - Eto, Hideaki

AU - Tinto, Halidou

AU - Karema, Corine

AU - D'Alessandro, Umberto

AU - do Rosario, Virgilio

AU - Kobayakawa, Takatoshi

AU - Ntoumi, Francine

AU - Carter, Richard

AU - Tanabe, Kazuyuki

PY - 2008/9/11

Y1 - 2008/9/11

N2 - Background: Plasmodium vivax is estimated to affect 75 million people annually. It is reportedly absent, however, from west and central Africa due to the high prevalence of the Duffy negative phenotype in the indigenous populations. Despite this, non-African travellers consistently return to their own countries with P. vivax malaria after visiting this region. An attempt was made, therefore, to detect the presence of P. vivax parasites in blood samples collected from the indigenous populations of west and central Africa.Methods: Parasite species typing (for all four human malaria parasites) was carried out by PCR on 2,588 blood samples collected from individuals from nine African malaria-endemic countries.Results: Most infections (98.5%) were Plasmodium falciparum, Plasmodium malariae was identified in 8.5% of all infections, and Plasmodium ovale in 3.9%. The prevalence of both parasites varied greatly by country. Only one case of P. vivax was detected from Sao Tome, an island off the west coast of Africa, confirming the scarcity of this parasite in Africa.Conclusion: The prevalence of P. vivax in local populations in sub-Saharan Africa is very low, despite the frequent identification of this parasite in non-African travellers.

AB - Background: Plasmodium vivax is estimated to affect 75 million people annually. It is reportedly absent, however, from west and central Africa due to the high prevalence of the Duffy negative phenotype in the indigenous populations. Despite this, non-African travellers consistently return to their own countries with P. vivax malaria after visiting this region. An attempt was made, therefore, to detect the presence of P. vivax parasites in blood samples collected from the indigenous populations of west and central Africa.Methods: Parasite species typing (for all four human malaria parasites) was carried out by PCR on 2,588 blood samples collected from individuals from nine African malaria-endemic countries.Results: Most infections (98.5%) were Plasmodium falciparum, Plasmodium malariae was identified in 8.5% of all infections, and Plasmodium ovale in 3.9%. The prevalence of both parasites varied greatly by country. Only one case of P. vivax was detected from Sao Tome, an island off the west coast of Africa, confirming the scarcity of this parasite in Africa.Conclusion: The prevalence of P. vivax in local populations in sub-Saharan Africa is very low, despite the frequent identification of this parasite in non-African travellers.

U2 - 10.1186/1475-2875-7-174

DO - 10.1186/1475-2875-7-174

M3 - Article

VL - 7

SP - -

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

M1 - 174

ER -