FAK regulates IL-33 expression by controlling chromatin accessibility at c-Jun motifs

Billie Griffith, Rosanna Upstill-Goddard, Holly Brunton, Graeme R Grimes, Andrew V Biankin, Bryan Serrels, Adam Byron, Margaret C Frame

Research output: Contribution to journalArticlepeer-review

Abstract

Focal adhesion kinase (FAK) localizes to focal adhesions and is overexpressed in many cancers. FAK can also translocate to the nucleus, where it binds to, and regulates, several transcription factors, including MBD2, p53 and IL-33, to control gene expression by unknown mechanisms. We have used ATAC-seq to reveal that FAK controls chromatin accessibility at a subset of regulated genes. Integration of ATAC-seq and RNA-seq data showed that FAK-dependent chromatin accessibility is linked to differential gene expression, including of the FAK-regulated cytokine and transcriptional regulator interleukin-33 (Il33), which controls anti-tumor immunity. Analysis of the accessibility peaks on the Il33 gene promoter/enhancer regions revealed sequences for several transcription factors, including ETS and AP-1 motifs, and we show that c-Jun, a component of AP-1, regulates Il33 gene expression by binding to its enhancer in a FAK kinase-dependent manner. This work provides the first demonstration that FAK controls transcription via chromatin accessibility, identifying a novel mechanism by which nuclear FAK regulates biologically important gene expression.
Original languageEnglish
JournalScientific Reports
Early online date8 Jan 2021
DOIs
Publication statusE-pub ahead of print - 8 Jan 2021

Keywords

  • FAK
  • c-Jun
  • IL-33
  • chromatin accessability

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