FAK-Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique

Guangping Tai, Peter Hohenstein, Jamie A Davies

Research output: Contribution to journalArticlepeer-review

Abstract

This report describes a hierarchical screening technique for identification of pathways that control the morphogenesis of the renal collecting duct system. The multi-step screen involves a first round using a 2-dimensional, cell-line-based scrape-healing assay, then a second round using a 3-dimensional tubulogenesis assay; both of these rounds use new cell lines described in this report. The final stage is ex vivo organ culture. We demonstrate the utility of the screen by using it to identify the FAK-Src-pathway signalling as being important for collecting duct development, specifically for the cell proliferation on which this development depends.
Original languageEnglish
Pages (from-to)416-23
Number of pages8
JournalBiology Open
Volume2
Issue number4
DOIs
Publication statusPublished - 15 Apr 2013

Fingerprint

Dive into the research topics of 'FAK-Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique'. Together they form a unique fingerprint.

Cite this