FAK-Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique

Guangping Tai; Peter Hohenstein; Jamie A Davies

doi:10.1242/bio.20133780

FAK-Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique

Guangping Tai, Peter Hohenstein, Jamie A Davies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

This report describes a hierarchical screening technique for identification of pathways that control the morphogenesis of the renal collecting duct system. The multi-step screen involves a first round using a 2-dimensional, cell-line-based scrape-healing assay, then a second round using a 3-dimensional tubulogenesis assay; both of these rounds use new cell lines described in this report. The final stage is ex vivo organ culture. We demonstrate the utility of the screen by using it to identify the FAK-Src-pathway signalling as being important for collecting duct development, specifically for the cell proliferation on which this development depends.

Original language	English
Pages (from-to)	416-23
Number of pages	8
Journal	Biology Open
Volume	2
Issue number	4
DOIs	https://doi.org/10.1242/bio.20133780
Publication status	Published - 15 Apr 2013

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10.1242/bio.20133780

FAK-Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique
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http://bio.biologists.org/content/2/4/416

Reducing mouse no complex genetic exper
Hohenstein, P.
UK central government bodies/local authorities, health and hospital authorities
1/10/12 → 9/07/14
Project: Research
Control of development and reproductive traits
Burdon, T., Argyle, D., Ashworth, C., Beard, P., Brunton, P., Burt, D., Clinton, M., Dunn, I., Farquharson, C., Headon, D., Hocking, P., Hohenstein, P., Hume, D., Jackson, I., McColl, B., McGrew, M., McLachlan, G., Sang, H., Summers, K. & Whitelaw, B.
BBSRC
1/04/12 → 31/03/17
Project: Research
A tissue engineering approach to reduce animal use in renal development and renal organ replacement
Davies, J.
MRC
10/03/08 → 9/03/11
Project: Research

Cite this

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title = "FAK-Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique",

abstract = "This report describes a hierarchical screening technique for identification of pathways that control the morphogenesis of the renal collecting duct system. The multi-step screen involves a first round using a 2-dimensional, cell-line-based scrape-healing assay, then a second round using a 3-dimensional tubulogenesis assay; both of these rounds use new cell lines described in this report. The final stage is ex vivo organ culture. We demonstrate the utility of the screen by using it to identify the FAK-Src-pathway signalling as being important for collecting duct development, specifically for the cell proliferation on which this development depends.",

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AB - This report describes a hierarchical screening technique for identification of pathways that control the morphogenesis of the renal collecting duct system. The multi-step screen involves a first round using a 2-dimensional, cell-line-based scrape-healing assay, then a second round using a 3-dimensional tubulogenesis assay; both of these rounds use new cell lines described in this report. The final stage is ex vivo organ culture. We demonstrate the utility of the screen by using it to identify the FAK-Src-pathway signalling as being important for collecting duct development, specifically for the cell proliferation on which this development depends.

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FAK-Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique

Abstract / Description of output

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Projects

Reducing mouse no complex genetic exper

Control of development and reproductive traits

A tissue engineering approach to reduce animal use in renal development and renal organ replacement

Cite this