Fast diffusing p75NTR monomers support apoptosis and growth cone collapse by neurotrophin ligands

Laura Marchetti, Fulvio Bonsignore, Francesco Gobbo, Rosy Amodeo, Mariantonietta Calvello, Ajesh Jacob, Giovanni Signore, Chiara Schirripa Spagnolo, David Porciani, Marco Mainardi, Fabio Beltram, Stefano Luin, Antonino Cattaneo

Research output: Contribution to journalArticlepeer-review

Abstract

The p75 neurotrophin (NT) receptor (p75NTR) plays a crucial role in balancing survival-versus-death decisions in the nervous system. Yet, despite 2 decades of structural and biochemical studies, a comprehensive, accepted model for p75NTR activation by NT ligands is still missing. Here, we present a single-molecule study of membrane p75NTR in living cells, demonstrating that the vast majority of receptors are monomers before and after NT activation. Interestingly, the stoichiometry and diffusion properties of the wild-type (wt) p75NTR are almost identical to those of a receptor mutant lacking residues previously believed to induce oligomerization. The wt p75NTR and mutated (mut) p75NTR differ in their partitioning in cholesterol-rich membrane regions upon nerve growth factor (NGF) stimulation: We argue that this is the origin of the ability of wt p75NTR , but not of mut p75NTR, to mediate immature NT (proNT)-induced apoptosis. Both p75NTR forms support proNT-induced growth cone retraction: We show that receptor surface accumulation is the driving force for cone collapse. Overall, our data unveil the multifaceted activity of the p75NTR monomer and let us provide a coherent interpretative frame of existing conflicting data in the literature.
Original languageEnglish
Pages (from-to)21563-21572
JournalProceedings of the National Academy of Sciences
Volume116
Issue number43
Early online date12 Sep 2019
DOIs
Publication statusPublished - 22 Oct 2019

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