TY - UNPB
T1 - FAST-STEM: Synthetic Biology Toolkit for Next Generation Regenerative Medicine
AU - Rosenstein, Aaron H.
AU - Chai, Andrew
AU - Gulati, Nitya
AU - Yin, Ting
AU - Nguyen, Maria
AU - Serra, Danielle
AU - Devina, Tania
AU - Gandhi, Aanshi
AU - Saleh, Mohammad
AU - Gilbert, Penney
AU - Laflamme, Michael A.
AU - Ogawa, Shinichiro
AU - Muffat, Julien
AU - Li, Yun
AU - Protze, Stephanie
AU - Kunath, Tilo
AU - Administrator, Sneak Peek
AU - Garton, Michael
AU - Sambathkumar, Rangarajan
AU - Murareanu, Brandon M.
AU - Dhaliwal, Navroop K.
AU - Sun, Fumao
AU - Zhao, Xinyaun
AU - Dadvar, Abolfazl
AU - Al-attar, Rasha
PY - 2024/7/24
Y1 - 2024/7/24
N2 - Very recent clinical advancements in human stem cell (hPSC)
based tissue replacement have opened a new frontier of possibility:
therapeutically augmented human cell engraftment. Therapeutic augmentation
requires the design of synthetic gene circuits that are compatible with hPSCs.
Such design is highly challenging due to: (a) poor translation of circuits
developed in immortalized cell lines; (b) absence of effective tools for
synthetic human stem cell development. To address this, we developed FAST-STEM
(Facile Accelerated Stem-cell Transgene integration with SynBio Tunable
Engineering Modes), a platform that enables design–build–test–learn style hPSC
engineering. FAST-STEM facilitates fast iterative synthetic cell evolution, zygosity
manipulation, and highly controlled library screening. Differentiation ready synthetic
stem cells can be generated an-order-of-magnitude faster by FAST-STEM than by
current site-specific methods. We demonstrate FAST-STEM potential to revolutionize
human synthetic biology, by creation of synthetic human liver, glial-cells, muscle,
cardiomyocytes, pancreatic beta-cells, cortical neurons, and dopaminergic neurons.
AB - Very recent clinical advancements in human stem cell (hPSC)
based tissue replacement have opened a new frontier of possibility:
therapeutically augmented human cell engraftment. Therapeutic augmentation
requires the design of synthetic gene circuits that are compatible with hPSCs.
Such design is highly challenging due to: (a) poor translation of circuits
developed in immortalized cell lines; (b) absence of effective tools for
synthetic human stem cell development. To address this, we developed FAST-STEM
(Facile Accelerated Stem-cell Transgene integration with SynBio Tunable
Engineering Modes), a platform that enables design–build–test–learn style hPSC
engineering. FAST-STEM facilitates fast iterative synthetic cell evolution, zygosity
manipulation, and highly controlled library screening. Differentiation ready synthetic
stem cells can be generated an-order-of-magnitude faster by FAST-STEM than by
current site-specific methods. We demonstrate FAST-STEM potential to revolutionize
human synthetic biology, by creation of synthetic human liver, glial-cells, muscle,
cardiomyocytes, pancreatic beta-cells, cortical neurons, and dopaminergic neurons.
U2 - 10.2139/ssrn.4903211
DO - 10.2139/ssrn.4903211
M3 - Preprint
T3 - CELL-D-24-02515
BT - FAST-STEM: Synthetic Biology Toolkit for Next Generation Regenerative Medicine
PB - Elsevier
CY - https://papers.ssrn.com/
ER -