Fate mapping of IL-17-producing T cells in inflammatory responses

K. Hirota, J.H. Duarte, M. Veldhoen, E. Hornsby, Y. Li, H. Ahlfors, C. Wilhelm, M. Tolaini, U. Menzel, A. Garefalaki, A.J. Potocnik, B. Stockinger, D.J. Cua

Research output: Contribution to journalArticlepeer-review

Abstract

Here we describe a reporter mouse strain designed to map the fate of cells that have activated interleukin 17A (IL-17A). We found that IL-17-producing helper T cells (T 17 cells) had distinct plasticity in different inflammatory settings. Chronic inflammatory conditions in experimental autoimmune encephalomyelitis (EAE) caused a switch to alternative cytokines in T 17 cells, whereas acute cutaneous infection with Candida albicans did not result in the deviation of T 17 cells to the production of alternative cytokines, although IL-17A production was shut off in the course of the infection. During the development of EAE, interferon-γ (IFN-γ) and other proinflammatory cytokines in the spinal cord were produced almost exclusively by cells that had produced IL-17 before their conversion by IL-23 ('ex-T 17 cells'). Thus, this model allows the actual functional fate of effector T cells to be related to T 17 developmental origin regardless of IL-17 expression.
Original languageEnglish
Pages (from-to)255-263
Number of pages9
JournalNature Immunology
Volume12
Issue number3
Early online date30 Jan 2011
DOIs
Publication statusPublished - 1 Mar 2011

Keywords

  • cytokine
  • gamma interferon
  • interleukin 17
  • interleukin 23
  • allergic encephalomyelitis
  • animal cell
  • animal experiment
  • animal model
  • animal tissue
  • article
  • candidiasis
  • CD4+ T lymphocyte
  • cell fate
  • controlled study
  • gene mapping
  • genetic transcription
  • helper cell
  • in vivo study
  • inflammation
  • mouse
  • nonhuman
  • priority journal
  • Animals
  • Encephalomyelitis, Autoimmune, Experimental
  • Flow Cytometry
  • Genes, Reporter
  • Inflammation
  • Interferon-gamma
  • Interleukin-17
  • Mice
  • Mice, Knockout
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction
  • T-Lymphocytes
  • T-helper 17 cells

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