Feline mammary carcinoma stem cells are tumorigenic, radioresistant, chemoresistant and defective in activation of the ATM/p53 DNA damage pathway

L Y Pang, Thalia Blacking, R W Else, A Sherman, H M Sang, B A Whitelaw, T R Hupp, D J Argyle

Research output: Contribution to journalArticlepeer-review

Abstract

Cancer stem cells were identified in a feline mammary carcinoma cell line by demonstrating expression of CD133 and utilising the tumour sphere assay. A population of cells was identified that had an invasive, mesenchymal phenotype, expressed markers of pluripotency and enhanced tumour formation in the NOD-SCID mouse and chick embryo models. This population of feline mammary carcinoma stem cells was resistant to chemotherapy and radiation, possibly due to aberrant activation of the ATM/p53 DNA damage pathway. Epithelial-mesenchymal transition was a feature of the invasive phenotype. These data demonstrate that cancer stem cells are a feature of mammary cancer in cats.
Original languageEnglish
Pages (from-to)414-423
Number of pages10
JournalVeterinary Journal
Volume196
Issue number3
Early online date7 Dec 2012
DOIs
Publication statusPublished - Jun 2013

Keywords

  • Feline mammary carcinoma
  • Cancer stem cell
  • p53
  • Epithelial-mesenchymal transition

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