Feline mammary carcinoma stem cells are tumorigenic, radioresistant, chemoresistant and defective in activation of the ATM/p53 DNA damage pathway

L Y Pang; Thalia Blacking; R W Else; A Sherman; H M Sang; B A Whitelaw; T R Hupp; D J Argyle

doi:10.1016/j.tvjl.2012.10.021

Feline mammary carcinoma stem cells are tumorigenic, radioresistant, chemoresistant and defective in activation of the ATM/p53 DNA damage pathway

L Y Pang, Thalia Blacking, R W Else, A Sherman, H M Sang, B A Whitelaw, T R Hupp, D J Argyle

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Cancer stem cells were identified in a feline mammary carcinoma cell line by demonstrating expression of CD133 and utilising the tumour sphere assay. A population of cells was identified that had an invasive, mesenchymal phenotype, expressed markers of pluripotency and enhanced tumour formation in the NOD-SCID mouse and chick embryo models. This population of feline mammary carcinoma stem cells was resistant to chemotherapy and radiation, possibly due to aberrant activation of the ATM/p53 DNA damage pathway. Epithelial-mesenchymal transition was a feature of the invasive phenotype. These data demonstrate that cancer stem cells are a feature of mammary cancer in cats.

Original language	English
Pages (from-to)	414-423
Number of pages	10
Journal	Veterinary Journal
Volume	196
Issue number	3
Early online date	7 Dec 2012
DOIs	https://doi.org/10.1016/j.tvjl.2012.10.021
Publication status	Published - Jun 2013

Keywords / Materials (for Non-textual outputs)

Feline mammary carcinoma
Cancer stem cell
p53
Epithelial-mesenchymal transition

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10.1016/j.tvjl.2012.10.021

Feline mammary carcinoma stem cells are tumorigenic, radioresistant, chemoresistant and defective in activation of the ATM p53 DNA damage pathway
Available under Open Access
Final published version, 1.96 MB

http://www.sciencedirect.com/science/article/pii/S109002331200456X

Characterisation of primitive stem cell-like canince cancer cells : identification of therapeutic targets through genomics
Argyle, D., Hupp, T. & Wallace, M.
BBSRC
17/03/08 → 16/03/11
Project: Research

Cite this

@article{ff7369b8d66642d1b806d5ff06b3b989,

title = "Feline mammary carcinoma stem cells are tumorigenic, radioresistant, chemoresistant and defective in activation of the ATM/p53 DNA damage pathway",

abstract = "Cancer stem cells were identified in a feline mammary carcinoma cell line by demonstrating expression of CD133 and utilising the tumour sphere assay. A population of cells was identified that had an invasive, mesenchymal phenotype, expressed markers of pluripotency and enhanced tumour formation in the NOD-SCID mouse and chick embryo models. This population of feline mammary carcinoma stem cells was resistant to chemotherapy and radiation, possibly due to aberrant activation of the ATM/p53 DNA damage pathway. Epithelial-mesenchymal transition was a feature of the invasive phenotype. These data demonstrate that cancer stem cells are a feature of mammary cancer in cats.",

keywords = "Feline mammary carcinoma, Cancer stem cell, p53, Epithelial-mesenchymal transition",

author = "Pang, {L Y} and Thalia Blacking and Else, {R W} and A Sherman and Sang, {H M} and Whitelaw, {B A} and Hupp, {T R} and Argyle, {D J}",

note = "The authors{\textquoteright} work is supported by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC).",

year = "2013",

month = jun,

doi = "10.1016/j.tvjl.2012.10.021",

language = "English",

volume = "196",

pages = "414--423",

journal = "Veterinary Journal",

issn = "1090-0233",

publisher = "Bailliere Tindall Ltd",

number = "3",

}

TY - JOUR

T1 - Feline mammary carcinoma stem cells are tumorigenic, radioresistant, chemoresistant and defective in activation of the ATM/p53 DNA damage pathway

AU - Pang, L Y

AU - Blacking, Thalia

AU - Else, R W

AU - Sherman, A

AU - Sang, H M

AU - Whitelaw, B A

AU - Hupp, T R

AU - Argyle, D J

N1 - The authors’ work is supported by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC).

PY - 2013/6

Y1 - 2013/6

N2 - Cancer stem cells were identified in a feline mammary carcinoma cell line by demonstrating expression of CD133 and utilising the tumour sphere assay. A population of cells was identified that had an invasive, mesenchymal phenotype, expressed markers of pluripotency and enhanced tumour formation in the NOD-SCID mouse and chick embryo models. This population of feline mammary carcinoma stem cells was resistant to chemotherapy and radiation, possibly due to aberrant activation of the ATM/p53 DNA damage pathway. Epithelial-mesenchymal transition was a feature of the invasive phenotype. These data demonstrate that cancer stem cells are a feature of mammary cancer in cats.

AB - Cancer stem cells were identified in a feline mammary carcinoma cell line by demonstrating expression of CD133 and utilising the tumour sphere assay. A population of cells was identified that had an invasive, mesenchymal phenotype, expressed markers of pluripotency and enhanced tumour formation in the NOD-SCID mouse and chick embryo models. This population of feline mammary carcinoma stem cells was resistant to chemotherapy and radiation, possibly due to aberrant activation of the ATM/p53 DNA damage pathway. Epithelial-mesenchymal transition was a feature of the invasive phenotype. These data demonstrate that cancer stem cells are a feature of mammary cancer in cats.

KW - Feline mammary carcinoma

KW - Cancer stem cell

KW - p53

KW - Epithelial-mesenchymal transition

U2 - 10.1016/j.tvjl.2012.10.021

DO - 10.1016/j.tvjl.2012.10.021

M3 - Article

C2 - 23219486

SN - 1090-0233

VL - 196

SP - 414

EP - 423

JO - Veterinary Journal

JF - Veterinary Journal

IS - 3

ER -

Feline mammary carcinoma stem cells are tumorigenic, radioresistant, chemoresistant and defective in activation of the ATM/p53 DNA damage pathway

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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Projects

Characterisation of primitive stem cell-like canince cancer cells : identification of therapeutic targets through genomics

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