Fetal and adult hematopoietic stem cells require beta1 integrin function for colonizing fetal liver, spleen, and bone marrow

A J Potocnik, C Brakebusch, R Fässler

Research output: Contribution to journalArticlepeer-review

Abstract

Homing of hematopoietic stem cells (HSCs) into hematopoietic organs is a prerequisite for the establishment of hematopoiesis during embryogenesis and after bone marrow transplantation. We show that beta1 integrin-deficient HSCs from the para-aortic splanchnopleura and the fetal blood had hematolymphoid differentiation potential in vitro and in fetal organ cultures but were unable to seed fetal and adult hematopoietic tissues. Adult beta1 integrin null HSCs isolated from mice carrying loxP-tagged beta1 integrin alleles and ablated for beta1 integrin expression by retroviral cre transduction failed to engraft irradiated recipient mice. Moreover, absence of beta1 integrin resulted in sequestration of HSCs in the circulation and their reduced adhesion to endothelioma cells. These findings define beta1 integrin as an essential adhesion receptor for the homing of HSCs.

Original languageEnglish
Pages (from-to)653-63
Number of pages11
JournalImmunity
Volume12
Issue number6
Publication statusPublished - Jun 2000

Keywords

  • Aging
  • Animals
  • Antigens, CD29
  • Bone Marrow
  • Cell Adhesion
  • Cell Differentiation
  • Cell Movement
  • Cell Survival
  • Colony-Forming Units Assay
  • Fetus
  • Hematopoiesis
  • Hematopoietic Stem Cells
  • Liver
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Organ Culture Techniques
  • Radiation Chimera
  • Spleen

Fingerprint

Dive into the research topics of 'Fetal and adult hematopoietic stem cells require beta1 integrin function for colonizing fetal liver, spleen, and bone marrow'. Together they form a unique fingerprint.

Cite this