FGF signalling through RAS/MAPK and PI3K pathways regulates cell movement and gene expression in the chicken primitive streak without affecting E-cadherin expression

Katharine M Hardy; Tatiana A Yatskievych; Jh Konieczka; Alexander S Bobbs; Parker B Antin

doi:10.1186/1471-213X-11-20

FGF signalling through RAS/MAPK and PI3K pathways regulates cell movement and gene expression in the chicken primitive streak without affecting E-cadherin expression

Katharine M Hardy, Tatiana A Yatskievych, Jh Konieczka, Alexander S Bobbs, Parker B Antin

Work enabled by Edinburgh Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

FGF signalling regulates numerous aspects of early embryo development. During gastrulation in amniotes, epiblast cells undergo an epithelial to mesenchymal transition (EMT) in the primitive streak to form the mesoderm and endoderm. In mice lacking FGFR1, epiblast cells in the primitive streak fail to downregulate E-cadherin and undergo EMT, and cell migration is inhibited. This study investigated how FGF signalling regulates cell movement and gene expression in the primitive streak of chicken embryos.

Original language	English
Pages (from-to)	20
Journal	Developmental Biology
Volume	11
DOIs	https://doi.org/10.1186/1471-213X-11-20
Publication status	Published - 2011

Keywords / Materials (for Non-textual outputs)

Animals
Blotting, Western
Cadherins
Cell Movement
Chick Embryo
Electroporation
Fibroblast Growth Factors
Gastrulation
Gene Expression
Gene Expression Regulation, Developmental
In Situ Hybridization
Microarray Analysis
Mitogen-Activated Protein Kinases
Phosphatidylinositol 3-Kinases
Polymerase Chain Reaction
Primitive Streak
Proto-Oncogene Proteins c-akt
Receptors, Fibroblast Growth Factor
Signal Transduction
Transcription Factors
ras Proteins

Access to Document

10.1186/1471-213X-11-20

FGF signalling through RAS:MAPK and PI3K pathways regulates cell movement and gene expression in the chicken primitive streak without affecting E-cadherin expression
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Final published version, 1.12 MB

Ark Genomics
Watson, M. (Principal Investigator), Archibald, A. (Co-investigator) & Burt, D. (Co-investigator)
Biotechnology and Biological Sciences Research Council
1/04/12 → 31/03/17
Project: Research

Cite this

@article{deb908e2783340418624704511414f3a,

title = "FGF signalling through RAS/MAPK and PI3K pathways regulates cell movement and gene expression in the chicken primitive streak without affecting E-cadherin expression",

abstract = "FGF signalling regulates numerous aspects of early embryo development. During gastrulation in amniotes, epiblast cells undergo an epithelial to mesenchymal transition (EMT) in the primitive streak to form the mesoderm and endoderm. In mice lacking FGFR1, epiblast cells in the primitive streak fail to downregulate E-cadherin and undergo EMT, and cell migration is inhibited. This study investigated how FGF signalling regulates cell movement and gene expression in the primitive streak of chicken embryos.",

keywords = "Animals, Blotting, Western, Cadherins, Cell Movement, Chick Embryo, Electroporation, Fibroblast Growth Factors, Gastrulation, Gene Expression, Gene Expression Regulation, Developmental, In Situ Hybridization, Microarray Analysis, Mitogen-Activated Protein Kinases, Phosphatidylinositol 3-Kinases, Polymerase Chain Reaction, Primitive Streak, Proto-Oncogene Proteins c-akt, Receptors, Fibroblast Growth Factor, Signal Transduction, Transcription Factors, ras Proteins",

author = "Hardy, \{Katharine M\} and Yatskievych, \{Tatiana A\} and Jh Konieczka and Bobbs, \{Alexander S\} and Antin, \{Parker B\}",

year = "2011",

doi = "10.1186/1471-213X-11-20",

language = "English",

volume = "11",

pages = "20",

journal = "Developmental Biology",

issn = "0012-1606",

publisher = "Elsevier",

}

TY - JOUR

T1 - FGF signalling through RAS/MAPK and PI3K pathways regulates cell movement and gene expression in the chicken primitive streak without affecting E-cadherin expression

AU - Hardy, Katharine M

AU - Yatskievych, Tatiana A

AU - Konieczka, Jh

AU - Bobbs, Alexander S

AU - Antin, Parker B

PY - 2011

Y1 - 2011

N2 - FGF signalling regulates numerous aspects of early embryo development. During gastrulation in amniotes, epiblast cells undergo an epithelial to mesenchymal transition (EMT) in the primitive streak to form the mesoderm and endoderm. In mice lacking FGFR1, epiblast cells in the primitive streak fail to downregulate E-cadherin and undergo EMT, and cell migration is inhibited. This study investigated how FGF signalling regulates cell movement and gene expression in the primitive streak of chicken embryos.

AB - FGF signalling regulates numerous aspects of early embryo development. During gastrulation in amniotes, epiblast cells undergo an epithelial to mesenchymal transition (EMT) in the primitive streak to form the mesoderm and endoderm. In mice lacking FGFR1, epiblast cells in the primitive streak fail to downregulate E-cadherin and undergo EMT, and cell migration is inhibited. This study investigated how FGF signalling regulates cell movement and gene expression in the primitive streak of chicken embryos.

KW - Animals

KW - Blotting, Western

KW - Cadherins

KW - Cell Movement

KW - Chick Embryo

KW - Electroporation

KW - Fibroblast Growth Factors

KW - Gastrulation

KW - Gene Expression

KW - Gene Expression Regulation, Developmental

KW - In Situ Hybridization

KW - Microarray Analysis

KW - Mitogen-Activated Protein Kinases

KW - Phosphatidylinositol 3-Kinases

KW - Polymerase Chain Reaction

KW - Primitive Streak

KW - Proto-Oncogene Proteins c-akt

KW - Receptors, Fibroblast Growth Factor

KW - Signal Transduction

KW - Transcription Factors

KW - ras Proteins

U2 - 10.1186/1471-213X-11-20

DO - 10.1186/1471-213X-11-20

M3 - Article

C2 - 21418646

SN - 0012-1606

VL - 11

SP - 20

JO - Developmental Biology

JF - Developmental Biology

ER -

FGF signalling through RAS/MAPK and PI3K pathways regulates cell movement and gene expression in the chicken primitive streak without affecting E-cadherin expression

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Projects

Ark Genomics

Cite this