Fine-structure molecular epidemiological analysis of Staphylococcus aureus recovered from cows

Ross Fitzgerald, W. J. Meaney, P. J. Hartigan, C. J. Smyth, V. Kapur

Research output: Contribution to journalArticlepeer-review


Sixty-three Staphylococcus aureus isolates recovered from bovine sources in the USA and the Republic of Ireland were characterized by multilocus enzyme electrophoresis (MLEE), ribotyping, and random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) typing at two separate laboratories. The S. aureus isolates were assigned by MLEE to 10 electrophoretic types (ETs) (Index of Discrimination, D = 0.779). In contrast, the same isolates were assigned to 13 ribotypes (D = 0.888), and to 12 RAPD types (D = 0.898). A common clone, ET3, of worldwide distribution, was represented by six distinct combinations of ribotypes and RAPD types. S. aureus clones recovered from cows in Ireland were also associated with mastitis in dairy cows in the USA. These findings are consistent with the hypothesis that only a few specialized clones of S. aureus are responsible for the majority of cases of bovine mastitis, and that these clones have a broad geographic distribution.
Original languageEnglish
Pages (from-to)261-9
Number of pages9
JournalEpidemiology & Infection
Issue number2
Publication statusPublished - Oct 1997


  • Animals
  • Cattle
  • DNA, Bacterial/genetics
  • Discriminant Analysis
  • Electrophoresis, Starch Gel
  • Female
  • Ireland/epidemiology
  • Mastitis, Bovine/epidemiology
  • Mastitis, Bovine/microbiology
  • Molecular Epidemiology
  • Random Amplified Polymorphic DNA Technique
  • Restriction Mapping
  • Sensitivity and Specificity
  • Staphylococcal Infections/epidemiology
  • Staphylococcal Infections/microbiology
  • Staphylococcal Infections/veterinary
  • Staphylococcus aureus/genetics
  • United States/epidemiology


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