First human hNT neurons patterned on parylene-C/silicon dioxide substrates: Combining an accessible cell line and robust patterning technology for the study of the pathological adult human brain

C. F. Unsworth, E. S. Graham, E. Delivopoulos, M. Dragunow, Alan Murray

Research output: Contribution to journalArticlepeer-review

Abstract

In this communication, we describe a new method which has enabled the first patterning of human neurons (derived from the human teratocarcinoma cell line (hNT)) on parylene-C/silicon dioxide substrates. We reveal the details of the nanofabrication processes, cell differentiation and culturing protocols necessary to successfully pattern hNT neurons which are each key aspects of this new method. The benefits in patterning human neurons on silicon chip using an accessible cell line and robust patterning technology are of widespread value. Thus, using a combined technology such as this will facilitate the detailed study of the pathological human brain at both the single cell and network level. (C) 2010 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)154-157
Number of pages4
JournalJournal of Neuroscience Methods
Volume194
Issue number1
DOIs
Publication statusPublished - 15 Dec 2010

Keywords

  • Cell patterning
  • hNT neuron
  • NTera2 cell line
  • NT2
  • Parylene-C
  • Human brain cell
  • Parylene
  • Silicon chip
  • On chip
  • CULTURE
  • GROWTH

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