Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatin

Alison L Pidoux, Eun Shik Choi, Johanna K R Abbott, Xingkun Liu, Alexander Kagansky, Araceli G Castillo, Georgina L Hamilton, William Richardson, Juri Rappsilber, Xiangwei He, Robin C Allshire

Research output: Contribution to journalArticlepeer-review

Abstract

The mechanisms ensuring specific incorporation of CENP-A at centromeres are poorly understood. Mis16 and Mis18 are required for CENP-A localization at centromeres and form a complex that is conserved from fission yeast to human. Fission yeast sim1 mutants that alleviate kinetochore domain silencing are defective in Scm3Sp, the ortholog of budding yeast Scm3Sc. Scm3Sp depends on Mis16/18 for its centromere localization and like them is recruited to centromeres in late anaphase. Importantly, Scm3Sp coaffinity purifies with CENP-ACnp1 and associates with CENP-ACnp1 in vitro, yet localizes independently of intact CENP-ACnp1 chromatin and is differentially released from chromatin. While Scm3Sc has been proposed to form a unique hexameric nucleosome with CENP-ACse4 and histone H4 at budding yeast point centromeres, we favor a model in which Scm3Sp acts as a CENP-ACnp1 receptor/assembly factor, cooperating with Mis16 and Mis18 to receive CENP-ACnp1 from the Sim3 escort and mediate assembly of CENP-ACnp1 into subkinetochore chromatin.
Original languageEnglish
Pages (from-to)299-311
Number of pages13
JournalMolecular Cell
Volume33
Issue number3
DOIs
Publication statusPublished - 13 Feb 2009

Keywords

  • Carrier Proteins
  • Cell Cycle
  • Cell Cycle Proteins
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Kinetochores
  • Mutation
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins

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