Flexible material formulations for 3D printing of ordered porous beds with applications in bioprocess engineering

Simone Dimartino*, Giuseppe Galindo Rodríguez, Ursula Simon, Mariachiara Conti, Sulaiman Sarwar, Selva Athi Narayanan, Qihao Jiang, Nick Christofi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Background

3D printing is revolutioning many industrial sectors and has the potential to enhance also the biotechnology and bioprocessing fields. Here, we propose a new flexible material formulation to 3D print support matrices with complex, perfectly ordered morphology and with tuneable properties to suit a range of applications in bioprocess engineering.
Findings

Supports were fabricated using functional monomers as the key ingredients, enabling matrices with bespoke chemistry, such as charged groups, chemical moieties for further functionalization, and hydrophobic/hydrophilic groups. Other ingredients, e.g. crosslinkers and porogens, can be employed to fabricate supports with diverse characteristics of their porous network, providing an opportunity to further regulate the mechanical and mass transfer properties of the supports. Through this approach, we fabricated and demonstrated the operation of Schoen gyroid columns with (I) positive and negative charges for ion exchange chromatography, (II) enzyme bioreactors with immobilized trypsin to catalyse hydrolysis, and (III) bacterial biofilm bioreactors for fuel desulphurization.
Conclusions

This study demonstrates a simple, cost-effective, and flexible fabrication of customized 3D printed supports for different biotechnology and bioengineering applications.
Original languageEnglish
Article number20
JournalBioresources and Bioprocessing
Volume9
Early online date12 Mar 2022
DOIs
Publication statusE-pub ahead of print - 12 Mar 2022

Keywords / Materials (for Non-textual outputs)

  • Biomaterials
  • Additive Manufacturing
  • Chromatography
  • immobilized enzyme bioreactor
  • Bacterial Biofilm Bioreactor

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