Flt-3 ligand, in combination with bovine granulocyte-macrophage colony-stimulating factor and interleukin-4, promotes the growth of bovine bone marrow derived dendritic cells

Jayne Hope, D. Werling, R A Collins, B Mertens, Chris J Howard

Research output: Contribution to journalArticlepeer-review

Abstract

Culture of bone marrow precursor cells with cytokines, including granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-4 (IL-4) and the tyrosine kinase receptor binding proteins Flt-3 ligand (Flt-3L) and stem-cell factor (SCF), has previously been shown, in both mouse and human, to result in the generation of large numbers of dendritic cells. We extend these findings to bovine dendritic cells. Culture of bovine bone marrow cells with GM-CSF, IL-4 and either Flt-3L or SCF enhanced the generation of low buoyant-density dendritic cells. However, only the addition of Flt-3L to cells cultured with GM-CSF and IL-4 was shown to increase the number of dendritic cells and induce the differentiation of dendritic cells with potent capacity to stimulate allogeneic T cells and resting CD4+ memory T cells. The effective ability to stimulate T cells was associated with the expression of major histocompatibility complex (MHC) class II molecules and CD80/86 by dendritic cells. Bovine bone marrow derived dendritic cells appeared to be exclusively of myeloid origin because they expressed the myeloid-related antigens CD14, MyD-1 and CD11b.
Original languageEnglish
Pages (from-to)60-6
Number of pages7
JournalScandinavian Journal of Immunology
Volume51
Issue number1
Publication statusPublished - Jan 2000

Keywords

  • Animals
  • Antigen Presentation
  • Antigens, CD/metabolism
  • Cattle
  • Cell Differentiation/drug effects
  • Cell Division/drug effects
  • Dendritic Cells/cytology
  • Dendritic Cells/drug effects
  • Dendritic Cells/immunology
  • Drug Interactions
  • Granulocyte-Macrophage Colony-Stimulating Factor/administration & dosage
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/drug effects
  • Hematopoietic Stem Cells/immunology
  • Humans
  • Interleukin-4/administration & dosage
  • Lymphocyte Culture Test, Mixed
  • Membrane Proteins/administration & dosage
  • Mice
  • Phenotype
  • Recombinant Proteins/administration & dosage
  • Stem Cell Factor/administration & dosage

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