Fluidification of entanglements by a DNA bending protein

Yair A. G. Fosado; Jamieson Howard; Simon Weir; Agnes Noy; Mark C Leake; Davide Michieletto

doi:10.1103/PhysRevLett.130.058203

Fluidification of entanglements by a DNA bending protein

Yair A. G. Fosado^*, Jamieson Howard, Simon Weir, Agnes Noy, Mark C Leake, Davide Michieletto

^*Corresponding author for this work

School of Physics and Astronomy

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

In spite of the nanoscale and single-molecule insights into nucleoid associated proteins (NAPs), their role in modulating the mesoscale viscoelasticity of entangled DNA has been overlooked so far. By combining microrheology and molecular dynamics simulation, we find that the abundant NAP “integration host factor” (IHF) lowers the viscosity of entangled λDNA 20-fold at physiological concentrations and stoichiometries. Our results suggest that IHF may play a previously unappreciated role in resolving DNA entanglements and in turn may be acting as a “genomic fluidizer” for bacterial genomes.

Original language	English
Article number	058203
Pages (from-to)	1-6
Number of pages	6
Journal	Physical Review Letters
Volume	130
Issue number	5
DOIs	https://doi.org/10.1103/PhysRevLett.130.058203
Publication status	Published - 3 Feb 2023

Keywords / Materials (for Non-textual outputs)

cond-mat.soft

Access to Document

10.1103/PhysRevLett.130.058203

2205.03510v3Accepted author manuscript, 4.77 MB

https://arxiv.org/abs/2205.03510Licence: Creative Commons: Attribution-ShareAlike (CC-BY-SA)

Topologically Active Polymers
Michieletto, D.
EU government bodies
1/01/21 → 31/12/25
Project: Research
Topologically Active Polymers
Michieletto, D.
Other (Learned Society)
1/01/21 → 31/12/25
Project: Research

Cite this

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title = "Fluidification of entanglements by a DNA bending protein",

abstract = "In spite of the nanoscale and single-molecule insights into nucleoid associated proteins (NAPs), their role in modulating the mesoscale viscoelasticity of entangled DNA has been overlooked so far. By combining microrheology and molecular dynamics simulation, we find that the abundant NAP “integration host factor” (IHF) lowers the viscosity of entangled λDNA 20-fold at physiological concentrations and stoichiometries. Our results suggest that IHF may play a previously unappreciated role in resolving DNA entanglements and in turn may be acting as a “genomic fluidizer” for bacterial genomes.",

keywords = "cond-mat.soft",

author = "Fosado, {Yair A. G.} and Jamieson Howard and Simon Weir and Agnes Noy and Leake, {Mark C} and Davide Michieletto",

note = "Funding Information: D. M. acknowledges the Royal Society and the European Research Council (Grant Agreement No. 947918, TAP) for funding. J. H., M. L. are supported by Leverhulme Trust (Grant No. RPG-2017-340) and BBSRC (Grant No. BB/P000746/1), and A. N., M. L. acknowledge support from EPSRC (Grant No. EP/N027639/1). Publisher Copyright: {\textcopyright} 2023 American Physical Society.",

year = "2023",

month = feb,

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doi = "10.1103/PhysRevLett.130.058203",

language = "English",

volume = "130",

pages = "1--6",

journal = "Physical Review Letters",

issn = "0031-9007",

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TY - JOUR

T1 - Fluidification of entanglements by a DNA bending protein

AU - Fosado, Yair A. G.

AU - Howard, Jamieson

AU - Weir, Simon

AU - Noy, Agnes

AU - Leake, Mark C

AU - Michieletto, Davide

N1 - Funding Information: D. M. acknowledges the Royal Society and the European Research Council (Grant Agreement No. 947918, TAP) for funding. J. H., M. L. are supported by Leverhulme Trust (Grant No. RPG-2017-340) and BBSRC (Grant No. BB/P000746/1), and A. N., M. L. acknowledge support from EPSRC (Grant No. EP/N027639/1). Publisher Copyright: © 2023 American Physical Society.

PY - 2023/2/3

Y1 - 2023/2/3

N2 - In spite of the nanoscale and single-molecule insights into nucleoid associated proteins (NAPs), their role in modulating the mesoscale viscoelasticity of entangled DNA has been overlooked so far. By combining microrheology and molecular dynamics simulation, we find that the abundant NAP “integration host factor” (IHF) lowers the viscosity of entangled λDNA 20-fold at physiological concentrations and stoichiometries. Our results suggest that IHF may play a previously unappreciated role in resolving DNA entanglements and in turn may be acting as a “genomic fluidizer” for bacterial genomes.

AB - In spite of the nanoscale and single-molecule insights into nucleoid associated proteins (NAPs), their role in modulating the mesoscale viscoelasticity of entangled DNA has been overlooked so far. By combining microrheology and molecular dynamics simulation, we find that the abundant NAP “integration host factor” (IHF) lowers the viscosity of entangled λDNA 20-fold at physiological concentrations and stoichiometries. Our results suggest that IHF may play a previously unappreciated role in resolving DNA entanglements and in turn may be acting as a “genomic fluidizer” for bacterial genomes.

KW - cond-mat.soft

U2 - 10.1103/PhysRevLett.130.058203

DO - 10.1103/PhysRevLett.130.058203

M3 - Article

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JO - Physical Review Letters

JF - Physical Review Letters

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ER -

Fluidification of entanglements by a DNA bending protein

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Keywords / Materials (for Non-textual outputs)

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Topologically Active Polymers

Topologically Active Polymers

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