Fluidification of entanglements by a DNA bending protein

Yair A. G. Fosado*, Jamieson Howard, Simon Weir, Agnes Noy, Mark C Leake, Davide Michieletto

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

In spite of the nanoscale and single-molecule insights into nucleoid associated proteins (NAPs), their role in modulating the mesoscale viscoelasticity of entangled DNA has been overlooked so far. By combining microrheology and molecular dynamics simulation, we find that the abundant NAP “integration host factor” (IHF) lowers the viscosity of entangled λDNA 20-fold at physiological concentrations and stoichiometries. Our results suggest that IHF may play a previously unappreciated role in resolving DNA entanglements and in turn may be acting as a “genomic fluidizer” for bacterial genomes.
Original languageEnglish
Article number058203
Pages (from-to)1-6
Number of pages6
JournalPhysical Review Letters
Issue number5
Publication statusPublished - 3 Feb 2023

Keywords / Materials (for Non-textual outputs)

  • cond-mat.soft


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