Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

Anna C. De Luca, Michael Mazilu, Andrew Riches, Simon Herrington, Kishan Dholakia*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution


The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.

Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems VIII
Publication statusPublished - 7 May 2010
EventAdvanced Biomedical and Clinical Diagnostic Systems VIII - San Francisco, CA, United States
Duration: 24 Jan 201026 Jan 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceAdvanced Biomedical and Clinical Diagnostic Systems VIII
CountryUnited States
CitySan Francisco, CA


  • Fluorescence-free
  • PCA
  • Raman spectroscopy


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