Fluorescent Dye Cocktail for Multiplex Drug-Site Mapping on Human Serum Albumin

Jun Cheng Er, Marc Vendrell, Mui Kee Tang, Duanting Zhai, Young-Tae Chang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites.

Original languageEnglish
Pages (from-to)452-457
Number of pages6
JournalACS Combinatorial Science
Volume15
Issue number9
DOIs
Publication statusPublished - Sep 2013

Keywords

  • drug discovery
  • fluorescence
  • high-throughput screening human serum albumin
  • multiplex assays
  • pharmacokinetics
  • LIGAND-BINDING PROPERTIES
  • BODIPY LIBRARY
  • CO-BINDING
  • F-19 NMR
  • PROBE
  • SPECIFICITY
  • SENSOR

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