Fluorescent Dye Cocktail for Multiplex Drug-Site Mapping on Human Serum Albumin

Jun Cheng Er; Marc Vendrell; Mui Kee Tang; Duanting Zhai; Young-Tae Chang

doi:10.1021/co400060b

Fluorescent Dye Cocktail for Multiplex Drug-Site Mapping on Human Serum Albumin

Jun Cheng Er, Marc Vendrell, Mui Kee Tang, Duanting Zhai, Young-Tae Chang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites.

Original language	English
Pages (from-to)	452-457
Number of pages	6
Journal	ACS Combinatorial Science
Volume	15
Issue number	9
DOIs	https://doi.org/10.1021/co400060b
Publication status	Published - Sept 2013

Keywords / Materials (for Non-textual outputs)

drug discovery
fluorescence
high-throughput screening human serum albumin
multiplex assays
pharmacokinetics
LIGAND-BINDING PROPERTIES
BODIPY LIBRARY
CO-BINDING
F-19 NMR
PROBE
SPECIFICITY
SENSOR

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10.1021/co400060b

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title = "Fluorescent Dye Cocktail for Multiplex Drug-Site Mapping on Human Serum Albumin",

abstract = "Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites.",

keywords = "drug discovery, fluorescence, high-throughput screening human serum albumin, multiplex assays, pharmacokinetics, LIGAND-BINDING PROPERTIES, BODIPY LIBRARY, CO-BINDING, F-19 NMR, PROBE, SPECIFICITY, SENSOR",

author = "Er, {Jun Cheng} and Marc Vendrell and Tang, {Mui Kee} and Duanting Zhai and Young-Tae Chang",

year = "2013",

month = sep,

doi = "10.1021/co400060b",

language = "English",

volume = "15",

pages = "452--457",

journal = "ACS Combinatorial Science",

issn = "2156-8952",

publisher = "American Chemical Society",

number = "9",

}

TY - JOUR

T1 - Fluorescent Dye Cocktail for Multiplex Drug-Site Mapping on Human Serum Albumin

AU - Er, Jun Cheng

AU - Vendrell, Marc

AU - Tang, Mui Kee

AU - Zhai, Duanting

AU - Chang, Young-Tae

PY - 2013/9

Y1 - 2013/9

N2 - Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites.

AB - Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites.

KW - drug discovery

KW - fluorescence

KW - high-throughput screening human serum albumin

KW - multiplex assays

KW - pharmacokinetics

KW - LIGAND-BINDING PROPERTIES

KW - BODIPY LIBRARY

KW - CO-BINDING

KW - F-19 NMR

KW - PROBE

KW - SPECIFICITY

KW - SENSOR

U2 - 10.1021/co400060b

DO - 10.1021/co400060b

M3 - Article

SN - 2156-8952

VL - 15

SP - 452

EP - 457

JO - ACS Combinatorial Science

JF - ACS Combinatorial Science

IS - 9

ER -

Fluorescent Dye Cocktail for Multiplex Drug-Site Mapping on Human Serum Albumin

Abstract

Keywords / Materials (for Non-textual outputs)

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