FM-Sim: Protocol Definition, Simulation and Rate Inference for Neuroscience Assays

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Synaptic vesicle recycling at the presynaptic terminal of neurons is essential for the maintenance of neurotransmission at central synapses. Among the tools used to visualise the mechanics of this process is time-series fluorescence microscopy. Fluorescent dyes such as FM1-43, or engineered fluorescent versions of synaptic vesicle proteins such as pHluorins, have been employed to reveal different steps of this key process [3,7]. Predictive in silico modelling of potential experimental outcomes would be highly informative for these time consuming and expensive studies.
We present FM-Sim [9], user-friendly software for defining and simulating fluorescence microscopy experimental assays, with the following features: intuitive user definition of experimental protocols; automatic conversion of protocol definitions into time series rate value changes; domain-specific simulation model of a synaptic terminal; experimental data used for model parameter value inference; automatic Bayesian inference of parameter values [1,5] and reduction of inferred parameter set size for Bayesian inference.
Original languageEnglish
Title of host publicationComputational Methods in Systems Biology
Subtitle of host publication12th International Conference, CMSB 2014, Manchester, UK, November 17-19, 2014, Proceedings
PublisherSpringer International Publishing
Pages248-251
Number of pages4
Volume8859
ISBN (Electronic)978-3-319-12982-2
ISBN (Print)978-3-319-12981-5
DOIs
Publication statusPublished - 2014

Publication series

NameLecture Notes in Computer Science
Volume8859
ISSN (Print)0302-9743

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