FMRP Interacts with C/D Box snoRNA in the Nucleus and Regulates Ribosomal RNA Methylation

Michelle Ninochka D'Souza; Naveen Kumar Chandappa Gowda; Vishal Tiwari; Rosana Ottakandathil Babu; Praveen Anand; Sudhriti Ghosh Dastidar; Randhir Singh; Bhuvaneish Selvaraja; Rakhi Pal; Arati Ramesh; Sumantra Chattarji; Siddharthan Chandran; Akash Gulyani; Dasaradhi Palakodeti; Ravi S Muddashetty

doi:10.1016/j.isci.2018.11.007

FMRP Interacts with C/D Box snoRNA in the Nucleus and Regulates Ribosomal RNA Methylation

Michelle Ninochka D'Souza, Naveen Kumar Chandappa Gowda, Vishal Tiwari, Rosana Ottakandathil Babu, Praveen Anand, Sudhriti Ghosh Dastidar, Randhir Singh, Bhuvaneish Selvaraja, Rakhi Pal, Arati Ramesh, Sumantra Chattarji, Siddharthan Chandran, Akash Gulyani, Dasaradhi Palakodeti, Ravi S Muddashetty

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Abstract

FMRP is an RNA-binding protein that is known to localize in the cytoplasm and in the nucleus. Here, we have identified an interaction of FMRP with a specific set of C/D box snoRNAs in the nucleus. C/D box snoRNAs guide 2'O methylations of ribosomal RNA (rRNA) on defined sites, and this modification regulates rRNA folding and assembly of ribosomes. 2'O methylation of rRNA is partial on several sites in human embryonic stem cells, which results in ribosomes with differential methylation patterns. FMRP-snoRNA interaction affects rRNA methylation on several of these sites, and in the absence of FMRP, differential methylation pattern of rRNA is significantly altered. We found that FMRP recognizes ribosomes carrying specific methylation patterns on rRNA and the recognition of methylation pattern by FMRP may potentially determine the translation status of its target mRNAs. Thus, FMRP integrates its function in the nucleus and in the cytoplasm.

Original language	English
Pages (from-to)	399-411
Number of pages	13
Journal	iScience
Volume	9
Early online date	7 Nov 2018
DOIs	https://doi.org/10.1016/j.isci.2018.11.007
Publication status	Published - 30 Nov 2018

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10.1016/j.isci.2018.11.007

Chandran S FMRP interacts with...
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D'Souza, M. N., Gowda, N. K. C., Tiwari, V., Babu, R. O., Anand, P., Dastidar, S. G., Singh, R., Selvaraja, B., Pal, R., Ramesh, A., Chattarji, S., Chandran, S., Gulyani, A., Palakodeti, D., & Muddashetty, R. S. (2018). FMRP Interacts with C/D Box snoRNA in the Nucleus and Regulates Ribosomal RNA Methylation. iScience, 9, 399-411. https://doi.org/10.1016/j.isci.2018.11.007

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title = "FMRP Interacts with C/D Box snoRNA in the Nucleus and Regulates Ribosomal RNA Methylation",

abstract = "FMRP is an RNA-binding protein that is known to localize in the cytoplasm and in the nucleus. Here, we have identified an interaction of FMRP with a specific set of C/D box snoRNAs in the nucleus. C/D box snoRNAs guide 2'O methylations of ribosomal RNA (rRNA) on defined sites, and this modification regulates rRNA folding and assembly of ribosomes. 2'O methylation of rRNA is partial on several sites in human embryonic stem cells, which results in ribosomes with differential methylation patterns. FMRP-snoRNA interaction affects rRNA methylation on several of these sites, and in the absence of FMRP, differential methylation pattern of rRNA is significantly altered. We found that FMRP recognizes ribosomes carrying specific methylation patterns on rRNA and the recognition of methylation pattern by FMRP may potentially determine the translation status of its target mRNAs. Thus, FMRP integrates its function in the nucleus and in the cytoplasm.",

author = "D'Souza, {Michelle Ninochka} and Gowda, {Naveen Kumar Chandappa} and Vishal Tiwari and Babu, {Rosana Ottakandathil} and Praveen Anand and Dastidar, {Sudhriti Ghosh} and Randhir Singh and Bhuvaneish Selvaraja and Rakhi Pal and Arati Ramesh and Sumantra Chattarji and Siddharthan Chandran and Akash Gulyani and Dasaradhi Palakodeti and Muddashetty, {Ravi S}",

year = "2018",

month = nov,

day = "30",

doi = "10.1016/j.isci.2018.11.007",

language = "English",

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T1 - FMRP Interacts with C/D Box snoRNA in the Nucleus and Regulates Ribosomal RNA Methylation

AU - D'Souza, Michelle Ninochka

AU - Gowda, Naveen Kumar Chandappa

AU - Tiwari, Vishal

AU - Babu, Rosana Ottakandathil

AU - Anand, Praveen

AU - Dastidar, Sudhriti Ghosh

AU - Singh, Randhir

AU - Selvaraja, Bhuvaneish

AU - Pal, Rakhi

AU - Ramesh, Arati

AU - Chattarji, Sumantra

AU - Chandran, Siddharthan

AU - Gulyani, Akash

AU - Palakodeti, Dasaradhi

AU - Muddashetty, Ravi S

PY - 2018/11/30

Y1 - 2018/11/30

N2 - FMRP is an RNA-binding protein that is known to localize in the cytoplasm and in the nucleus. Here, we have identified an interaction of FMRP with a specific set of C/D box snoRNAs in the nucleus. C/D box snoRNAs guide 2'O methylations of ribosomal RNA (rRNA) on defined sites, and this modification regulates rRNA folding and assembly of ribosomes. 2'O methylation of rRNA is partial on several sites in human embryonic stem cells, which results in ribosomes with differential methylation patterns. FMRP-snoRNA interaction affects rRNA methylation on several of these sites, and in the absence of FMRP, differential methylation pattern of rRNA is significantly altered. We found that FMRP recognizes ribosomes carrying specific methylation patterns on rRNA and the recognition of methylation pattern by FMRP may potentially determine the translation status of its target mRNAs. Thus, FMRP integrates its function in the nucleus and in the cytoplasm.

AB - FMRP is an RNA-binding protein that is known to localize in the cytoplasm and in the nucleus. Here, we have identified an interaction of FMRP with a specific set of C/D box snoRNAs in the nucleus. C/D box snoRNAs guide 2'O methylations of ribosomal RNA (rRNA) on defined sites, and this modification regulates rRNA folding and assembly of ribosomes. 2'O methylation of rRNA is partial on several sites in human embryonic stem cells, which results in ribosomes with differential methylation patterns. FMRP-snoRNA interaction affects rRNA methylation on several of these sites, and in the absence of FMRP, differential methylation pattern of rRNA is significantly altered. We found that FMRP recognizes ribosomes carrying specific methylation patterns on rRNA and the recognition of methylation pattern by FMRP may potentially determine the translation status of its target mRNAs. Thus, FMRP integrates its function in the nucleus and in the cytoplasm.

U2 - 10.1016/j.isci.2018.11.007

DO - 10.1016/j.isci.2018.11.007

M3 - Article

C2 - 30469012

VL - 9

SP - 399

EP - 411

JO - iScience

JF - iScience

SN - 2589-0042

ER -

FMRP Interacts with C/D Box snoRNA in the Nucleus and Regulates Ribosomal RNA Methylation

Abstract

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