Fold or hold: experimental evolution in vitro

S. Collins, A. Rambaut, S.J. Bridgett

Research output: Contribution to journalArticlepeer-review


We introduce a system for experimental evolution consisting of populations of short oligonucleotides (Oli populations) evolving in a modified quantitative polymerase chain reaction (qPCR). It is tractable at the genetic, genomic, phenotypic and fitness levels. The Oli system uses DNA hairpins designed to form structures that self-prime under defined conditions. Selection acts on the phenotype of self-priming, after which differences in fitness are amplified and quantified using qPCR. We outline the methodological and bioinformatics tools for the Oli system here and demonstrate that it can be used as a conventional experimental evolution model system by test-driving it in an experiment investigating adaptive evolution under different rates of environmental change.
Original languageEnglish
Pages (from-to)2123-2134
Number of pages12
JournalJournal of Evolutionary Biology
Issue number10
Publication statusPublished - Oct 2013


  • adaptation
  • aptamer
  • DNA hairpin
  • experimental evolution
  • polymerase chain reaction


Dive into the research topics of 'Fold or hold: experimental evolution in vitro'. Together they form a unique fingerprint.

Cite this