Frequency and phenotype of NK cells and NK cell subsets in bovine lymphoid compartments and blood

Carly Hamilton; Suman Mahan; Charlotte Bell; Bernardo Villarreal-Ramos; Bryan Charleston; Gary Entrican; Jayne Hope

doi:10.1111/imm.12708

Frequency and phenotype of NK cells and NK cell subsets in bovine lymphoid compartments and blood

Carly Hamilton, Suman Mahan, Charlotte Bell, Bernardo Villarreal-Ramos, Bryan Charleston, Gary Entrican, Jayne Hope

Research output: Contribution to journal › Article › peer-review

Abstract

NK cells are widely distributed in lymphoid and non-lymphoid tissues however little is known about the recirculation of NK cells between blood and tissues. This is relevant to understanding recirculation in the steady-state and also for determining the roles for NK cells in vaccine-induced immunity and responses to infection. Therefore, the percentage of NK cells and their phenotype across peripheral blood, afferent lymph and lymph nodes in steady-state conditions was investigated in cattle using the pseudo-afferent lymphatic cannulation model. CD2+ CD25lo NK cells were the predominant subset of NK cells within the blood. In contrast, CD2- CD25hi NK cells were the main subset present within the skin-draining afferent lymphatic vessels and lymph nodes, indicating that CD2- NK cells are the principal NK cell subset trafficking to LNs via the afferent lymphatic vessel. Furthermore, a low percentage of NK cells were present in efferent lymph which were predominantly of the CD2- subset indicating that NK cells can egress from lymph nodes and return to circulation in steady-state conditions. These compartmentalisation data indicate that NK cells represent a population of recirculating lymphocytes in steady-state conditions and therefore may be important during immune responses to vaccination or infection.

Original language	English
Pages (from-to)	89-97
Journal	Immunology
Volume	151
Issue number	1
Early online date	7 Jan 2017
DOIs	https://doi.org/10.1111/imm.12708
Publication status	Published - May 2017

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10.1111/imm.12708

Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood
© 2017 The Authors. Immunology Published by John Wiley & Sons Ltd., Immunology This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Final published version, 844 KBLicence: Creative Commons: Attribution (CC-BY)

Innate immunity and endemic diseases in livestock species
Collie, D. (Principal Investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Fitzgerald, R. (Co-investigator), Freeman, T. (Co-investigator), Gally, D. (Co-investigator), Gill, A. (Co-investigator), Glass, E. (Co-investigator), Hocking, P. (Co-investigator), Hope, J. (Co-investigator), Hume, D. (Co-investigator), Kaiser, P. (Co-investigator), Mabbott, N. (Co-investigator), McLachlan, G. (Co-investigator), Morrison, L. (Co-investigator), Stevens, J. (Co-investigator), Stevens, M. (Co-investigator) & Watson, M. (Co-investigator)
Biotechnology and Biological Sciences Research Council
1/04/12 → 31/03/17
Project: Research
Neonatal vaccinations: role for innate immune cell interactions in BCG vaccination
Hope, J. (Principal Investigator)
Biotechnology and Biological Sciences Research Council
1/10/11 → 30/09/15
Project: Research

Jayne Hope
- jayne.hoperoslin.ed.acuk
- Royal (Dick) School of Veterinary Studies - Personal Chair of Immunology
- Edinburgh Imaging
Person: Academic: Research Active

Cite this

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title = "Frequency and phenotype of NK cells and NK cell subsets in bovine lymphoid compartments and blood",

abstract = "NK cells are widely distributed in lymphoid and non-lymphoid tissues however little is known about the recirculation of NK cells between blood and tissues. This is relevant to understanding recirculation in the steady-state and also for determining the roles for NK cells in vaccine-induced immunity and responses to infection. Therefore, the percentage of NK cells and their phenotype across peripheral blood, afferent lymph and lymph nodes in steady-state conditions was investigated in cattle using the pseudo-afferent lymphatic cannulation model. CD2+ CD25lo NK cells were the predominant subset of NK cells within the blood. In contrast, CD2- CD25hi NK cells were the main subset present within the skin-draining afferent lymphatic vessels and lymph nodes, indicating that CD2- NK cells are the principal NK cell subset trafficking to LNs via the afferent lymphatic vessel. Furthermore, a low percentage of NK cells were present in efferent lymph which were predominantly of the CD2- subset indicating that NK cells can egress from lymph nodes and return to circulation in steady-state conditions. These compartmentalisation data indicate that NK cells represent a population of recirculating lymphocytes in steady-state conditions and therefore may be important during immune responses to vaccination or infection. ",

author = "Carly Hamilton and Suman Mahan and Charlotte Bell and Bernardo Villarreal-Ramos and Bryan Charleston and Gary Entrican and Jayne Hope",

year = "2017",

month = may,

doi = "10.1111/imm.12708",

language = "English",

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journal = "Immunology",

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T1 - Frequency and phenotype of NK cells and NK cell subsets in bovine lymphoid compartments and blood

AU - Hamilton, Carly

AU - Mahan, Suman

AU - Bell, Charlotte

AU - Villarreal-Ramos, Bernardo

AU - Charleston, Bryan

AU - Entrican, Gary

AU - Hope, Jayne

PY - 2017/5

Y1 - 2017/5

N2 - NK cells are widely distributed in lymphoid and non-lymphoid tissues however little is known about the recirculation of NK cells between blood and tissues. This is relevant to understanding recirculation in the steady-state and also for determining the roles for NK cells in vaccine-induced immunity and responses to infection. Therefore, the percentage of NK cells and their phenotype across peripheral blood, afferent lymph and lymph nodes in steady-state conditions was investigated in cattle using the pseudo-afferent lymphatic cannulation model. CD2+ CD25lo NK cells were the predominant subset of NK cells within the blood. In contrast, CD2- CD25hi NK cells were the main subset present within the skin-draining afferent lymphatic vessels and lymph nodes, indicating that CD2- NK cells are the principal NK cell subset trafficking to LNs via the afferent lymphatic vessel. Furthermore, a low percentage of NK cells were present in efferent lymph which were predominantly of the CD2- subset indicating that NK cells can egress from lymph nodes and return to circulation in steady-state conditions. These compartmentalisation data indicate that NK cells represent a population of recirculating lymphocytes in steady-state conditions and therefore may be important during immune responses to vaccination or infection.

AB - NK cells are widely distributed in lymphoid and non-lymphoid tissues however little is known about the recirculation of NK cells between blood and tissues. This is relevant to understanding recirculation in the steady-state and also for determining the roles for NK cells in vaccine-induced immunity and responses to infection. Therefore, the percentage of NK cells and their phenotype across peripheral blood, afferent lymph and lymph nodes in steady-state conditions was investigated in cattle using the pseudo-afferent lymphatic cannulation model. CD2+ CD25lo NK cells were the predominant subset of NK cells within the blood. In contrast, CD2- CD25hi NK cells were the main subset present within the skin-draining afferent lymphatic vessels and lymph nodes, indicating that CD2- NK cells are the principal NK cell subset trafficking to LNs via the afferent lymphatic vessel. Furthermore, a low percentage of NK cells were present in efferent lymph which were predominantly of the CD2- subset indicating that NK cells can egress from lymph nodes and return to circulation in steady-state conditions. These compartmentalisation data indicate that NK cells represent a population of recirculating lymphocytes in steady-state conditions and therefore may be important during immune responses to vaccination or infection.

U2 - 10.1111/imm.12708

DO - 10.1111/imm.12708

M3 - Article

SN - 0019-2805

VL - 151

SP - 89

EP - 97

JO - Immunology

JF - Immunology

IS - 1

ER -

Frequency and phenotype of NK cells and NK cell subsets in bovine lymphoid compartments and blood

Abstract

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Innate immunity and endemic diseases in livestock species

Neonatal vaccinations: role for innate immune cell interactions in BCG vaccination

Profiles

Jayne Hope

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