Frugal fluorescence imaging of microbes: proof of concept for translation to point of care

Syam Mohan Padinjarottu Charinjathil Mohanan*, Beth Mills, Kay Russell, Sheelagh Duncan, Alex Kiang, Rachel Williams, N. Venkatesh Prajna, Kevin Dhaliwal, Gareth Williams

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Fluorescence imaging and ‘smart probes’ are an emerging point-of-care platform for microbial detection. This article proposes a proof-of-concept frugal fluorescence imaging system for the detection of bacteria such as Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. In this work, we investigate the capability of a trans-illuminating fluorescence imaging system to detect bacteria using a low-cost raspberry pi single board computer and a high-quality camera. This system is capable of producing high quality fluorescence and transmission images of bacterial samples with submicron resolution. We demonstrate the capabilities of the system to produce these images in conjunction with low-cost lens-based imaging optics.
Original languageEnglish
Title of host publicationSociety of Photo-Optical Instrumentation Engineers (SPIE)
Subtitle of host publicationSPIE Photonics West
Place of PublicationSan Francisco, California, United States
VolumeProceedings Volume PC11950,
Publication statusPublished - 7 Mar 2022


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