Frugal fluorescence imaging of microbes: proof of concept for translation to point of care

Syam Mohan Padinjarottu Charinjathil Mohanan; Beth Mills; Kay Russell; Sheelagh Duncan; Alex Kiang; Rachel Williams; N. Venkatesh  Prajna; Kevin Dhaliwal; Gareth Williams

doi:https://doi.org/10.1117/12.2609578

Frugal fluorescence imaging of microbes: proof of concept for translation to point of care

Syam Mohan Padinjarottu Charinjathil Mohanan^*, Beth Mills, Kay Russell, Sheelagh Duncan, Alex Kiang, Rachel Williams, N. Venkatesh Prajna, Kevin Dhaliwal, Gareth Williams

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract / Description of output

Fluorescence imaging and ‘smart probes’ are an emerging point-of-care platform for microbial detection. This article proposes a proof-of-concept frugal fluorescence imaging system for the detection of bacteria such as Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. In this work, we investigate the capability of a trans-illuminating fluorescence imaging system to detect bacteria using a low-cost raspberry pi single board computer and a high-quality camera. This system is capable of producing high quality fluorescence and transmission images of bacterial samples with submicron resolution. We demonstrate the capabilities of the system to produce these images in conjunction with low-cost lens-based imaging optics.

Original language	English
Title of host publication	Society of Photo-Optical Instrumentation Engineers (SPIE)
Subtitle of host publication	SPIE Photonics West
Place of Publication	San Francisco, California, United States
Publisher	SPIE
Volume	Proceedings Volume PC11950,
Edition	2022
DOIs	https://doi.org/10.1117/12.2609578
Publication status	Published - 7 Mar 2022

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Padinjarottu Charinjathil Mohanan, S. M., Mills, B., Russell, K., Duncan, S., Kiang, A., Williams, R., Prajna, N. V., Dhaliwal, K., & Williams, G. (2022). Frugal fluorescence imaging of microbes: proof of concept for translation to point of care. In Society of Photo-Optical Instrumentation Engineers (SPIE): SPIE Photonics West (2022 ed., Vol. Proceedings Volume PC11950,). Article 11950-13 SPIE. https://doi.org/10.1117/12.2609578

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title = "Frugal fluorescence imaging of microbes: proof of concept for translation to point of care",

abstract = "Fluorescence imaging and {\textquoteleft}smart probes{\textquoteright} are an emerging point-of-care platform for microbial detection. This article proposes a proof-of-concept frugal fluorescence imaging system for the detection of bacteria such as Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. In this work, we investigate the capability of a trans-illuminating fluorescence imaging system to detect bacteria using a low-cost raspberry pi single board computer and a high-quality camera. This system is capable of producing high quality fluorescence and transmission images of bacterial samples with submicron resolution. We demonstrate the capabilities of the system to produce these images in conjunction with low-cost lens-based imaging optics.",

author = "{Padinjarottu Charinjathil Mohanan}, {Syam Mohan} and Beth Mills and Kay Russell and Sheelagh Duncan and Alex Kiang and Rachel Williams and Prajna, {N. Venkatesh} and Kevin Dhaliwal and Gareth Williams",

year = "2022",

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Padinjarottu Charinjathil Mohanan, SM , Mills, B , Russell, K, Duncan, S, Kiang, A, Williams, R, Prajna, NV, Dhaliwal, K & Williams, G 2022, Frugal fluorescence imaging of microbes: proof of concept for translation to point of care. in Society of Photo-Optical Instrumentation Engineers (SPIE): SPIE Photonics West. 2022 edn, vol. Proceedings Volume PC11950, 11950-13, SPIE, San Francisco, California, United States. https://doi.org/10.1117/12.2609578

Frugal fluorescence imaging of microbes: proof of concept for translation to point of care. / Padinjarottu Charinjathil Mohanan, Syam Mohan ; Mills, Beth ; Russell, Kay et al.
Society of Photo-Optical Instrumentation Engineers (SPIE): SPIE Photonics West. Vol. Proceedings Volume PC11950, 2022. ed. San Francisco, California, United States: SPIE, 2022. 11950-13.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Frugal fluorescence imaging of microbes: proof of concept for translation to point of care

AU - Padinjarottu Charinjathil Mohanan, Syam Mohan

AU - Mills, Beth

AU - Russell, Kay

AU - Duncan, Sheelagh

AU - Kiang, Alex

AU - Williams, Rachel

AU - Prajna, N. Venkatesh

AU - Dhaliwal, Kevin

AU - Williams, Gareth

PY - 2022/3/7

Y1 - 2022/3/7

N2 - Fluorescence imaging and ‘smart probes’ are an emerging point-of-care platform for microbial detection. This article proposes a proof-of-concept frugal fluorescence imaging system for the detection of bacteria such as Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. In this work, we investigate the capability of a trans-illuminating fluorescence imaging system to detect bacteria using a low-cost raspberry pi single board computer and a high-quality camera. This system is capable of producing high quality fluorescence and transmission images of bacterial samples with submicron resolution. We demonstrate the capabilities of the system to produce these images in conjunction with low-cost lens-based imaging optics.

AB - Fluorescence imaging and ‘smart probes’ are an emerging point-of-care platform for microbial detection. This article proposes a proof-of-concept frugal fluorescence imaging system for the detection of bacteria such as Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. In this work, we investigate the capability of a trans-illuminating fluorescence imaging system to detect bacteria using a low-cost raspberry pi single board computer and a high-quality camera. This system is capable of producing high quality fluorescence and transmission images of bacterial samples with submicron resolution. We demonstrate the capabilities of the system to produce these images in conjunction with low-cost lens-based imaging optics.

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DO - https://doi.org/10.1117/12.2609578

M3 - Conference contribution

VL - Proceedings Volume PC11950,

BT - Society of Photo-Optical Instrumentation Engineers (SPIE)

PB - SPIE

CY - San Francisco, California, United States

ER -

Padinjarottu Charinjathil Mohanan SM , Mills B , Russell K, Duncan S, Kiang A, Williams R et al. Frugal fluorescence imaging of microbes: proof of concept for translation to point of care. In Society of Photo-Optical Instrumentation Engineers (SPIE): SPIE Photonics West. 2022 ed. Vol. Proceedings Volume PC11950,. San Francisco, California, United States: SPIE. 2022. 11950-13 doi: https://doi.org/10.1117/12.2609578

Frugal fluorescence imaging of microbes: proof of concept for translation to point of care

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