Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis

Kevin Ashelford; Maria E Eriksson; Christopher M Allen; Rosalinda D'Amore; Mikael Johansson; Peter Gould; Suzanne Kay; Andrew J Millar; Neil Hall; Anthony Hall

doi:10.1186/gb-2011-12-3-r28

Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis

Kevin Ashelford, Maria E Eriksson, Christopher M Allen, Rosalinda D'Amore, Mikael Johansson, Peter Gould, Suzanne Kay, Andrew J Millar, Neil Hall, Anthony Hall

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.

Original language	English
Pages (from-to)	R28
Journal	Genome Biology
Volume	12
Issue number	3
DOIs	https://doi.org/10.1186/gb-2011-12-3-r28
Publication status	Published - 2011

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10.1186/gb-2011-12-3-r28

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abstract = "Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.",

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AU - Ashelford, Kevin

AU - Eriksson, Maria E

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AU - D'Amore, Rosalinda

AU - Johansson, Mikael

AU - Gould, Peter

AU - Kay, Suzanne

AU - Millar, Andrew J

AU - Hall, Neil

AU - Hall, Anthony

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AB - Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.

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Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis

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Modelling circadian gene regulation in Arabidopsis thaliana

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Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis

Abstract / Description of output

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Modelling circadian gene regulation in Arabidopsis thaliana

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