Fumarate reductase: Structural and mechanistic insights from the catalytic reduction of 2-methylfumarate

Caroline Wardrope; Christopher Mowat; Malcolm Walkinshaw; Graeme Reid; Stephen Chapman

doi:10.1016/j.febslet.2006.02.016

Fumarate reductase: Structural and mechanistic insights from the catalytic reduction of 2-methylfumarate

Caroline Wardrope, Christopher Mowat, Malcolm Walkinshaw, Graeme Reid, Stephen Chapman

Research output: Contribution to journal › Article › peer-review

Abstract

The soluble fumarate reductase (FR) from Shewanella frigidimarina can catalyse the reduction of 2-methylfumarate with a kcat of 9.0 s−1 and a KM of 32 μM. This produces the chiral molecule 2-methylsuccinate. Here, we present the structure of FR to a resolution of 1.5 Å with 2-methylfumarate bound at the active site. The mode of binding of 2-methylfumarate allows us to predict the stereochemistry of the product as (S)-2-methylsuccinate. To test this prediction we have analysed the product stereochemistry by circular dichroism spectroscopy and confirmed the production of (S)-2-methylsuccinate.

Original language	English
Pages (from-to)	1677-1680
Number of pages	4
Journal	FEBS Letters
Volume	580
Issue number	6
Early online date	17 Feb 2006
DOIs	https://doi.org/10.1016/j.febslet.2006.02.016
Publication status	Published - 2006

Keywords / Materials (for Non-textual outputs)

Fumerate reductase
Flavocytochrome
c3
2-Methylfumerate
Mesaconate
2-Methylsuccinate

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10.1016/j.febslet.2006.02.016

http://www.sciencedirect.com/science/article/pii/S0014579306002031

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@article{941183abc550462fa9bc06a081bcc855,

title = "Fumarate reductase: Structural and mechanistic insights from the catalytic reduction of 2-methylfumarate",

abstract = "The soluble fumarate reductase (FR) from Shewanella frigidimarina can catalyse the reduction of 2-methylfumarate with a kcat of 9.0 s−1 and a KM of 32 μM. This produces the chiral molecule 2-methylsuccinate. Here, we present the structure of FR to a resolution of 1.5 {\AA} with 2-methylfumarate bound at the active site. The mode of binding of 2-methylfumarate allows us to predict the stereochemistry of the product as (S)-2-methylsuccinate. To test this prediction we have analysed the product stereochemistry by circular dichroism spectroscopy and confirmed the production of (S)-2-methylsuccinate.",

keywords = "Fumerate reductase, Flavocytochrome, c3, 2-Methylfumerate, Mesaconate, 2-Methylsuccinate",

author = "Caroline Wardrope and Christopher Mowat and Malcolm Walkinshaw and Graeme Reid and Stephen Chapman",

year = "2006",

doi = "10.1016/j.febslet.2006.02.016",

language = "English",

volume = "580",

pages = "1677--1680",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "6",

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TY - JOUR

T1 - Fumarate reductase: Structural and mechanistic insights from the catalytic reduction of 2-methylfumarate

AU - Wardrope, Caroline

AU - Mowat, Christopher

AU - Walkinshaw, Malcolm

AU - Reid, Graeme

AU - Chapman, Stephen

PY - 2006

Y1 - 2006

N2 - The soluble fumarate reductase (FR) from Shewanella frigidimarina can catalyse the reduction of 2-methylfumarate with a kcat of 9.0 s−1 and a KM of 32 μM. This produces the chiral molecule 2-methylsuccinate. Here, we present the structure of FR to a resolution of 1.5 Å with 2-methylfumarate bound at the active site. The mode of binding of 2-methylfumarate allows us to predict the stereochemistry of the product as (S)-2-methylsuccinate. To test this prediction we have analysed the product stereochemistry by circular dichroism spectroscopy and confirmed the production of (S)-2-methylsuccinate.

AB - The soluble fumarate reductase (FR) from Shewanella frigidimarina can catalyse the reduction of 2-methylfumarate with a kcat of 9.0 s−1 and a KM of 32 μM. This produces the chiral molecule 2-methylsuccinate. Here, we present the structure of FR to a resolution of 1.5 Å with 2-methylfumarate bound at the active site. The mode of binding of 2-methylfumarate allows us to predict the stereochemistry of the product as (S)-2-methylsuccinate. To test this prediction we have analysed the product stereochemistry by circular dichroism spectroscopy and confirmed the production of (S)-2-methylsuccinate.

KW - Fumerate reductase

KW - Flavocytochrome

KW - c3

KW - 2-Methylfumerate

KW - Mesaconate

KW - 2-Methylsuccinate

U2 - 10.1016/j.febslet.2006.02.016

DO - 10.1016/j.febslet.2006.02.016

M3 - Article

SN - 0014-5793

VL - 580

SP - 1677

EP - 1680

JO - FEBS Letters

JF - FEBS Letters

IS - 6

ER -

Fumarate reductase: Structural and mechanistic insights from the catalytic reduction of 2-methylfumarate

Abstract

Keywords / Materials (for Non-textual outputs)

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