Projects per year
contiguous pig reference genomes have recently been released. To support functional annotation of the pig genomes and
comparative analysis with large human transcriptomic datasets, we aimed to create a pig gene expression atlas. To achieve this
objective, we extended a previous approach developed for the chicken. We down-loaded RNAseq datasets from public repositories,
down-sampled to a common depth and quantified expression against a reference transcriptome using the mRNA quantitation tool,
Kallisto. We then used the network analysis tool, Graphia to identify clusters of transcripts that were co-expressed across the
merged dataset. Consistent with the principle of guilt-by-association, we identified co-expression clusters that were highly tissue
or cell-type restricted and contained transcription factors that have previously been implicated in lineage determination. Other
clusters were enriched for transcripts associated with biological processes, such as the cell cycle and oxidative phosphorylation.
The same approach was used to identify co-expression clusters within RNAseq data from multiple individual liver and brain
samples, highlighting cell type, process and region-specific gene expression. Evidence of conserved expression can add confidence
to assignment of orthology between pig and human genes. Many transcripts currently identified as novel genes with ENSSSCG or
LOC IDs were found to be co-expressed with annotated neighbouring transcripts in the same orientation indicating they may be
products of the same transcriptional unit. The meta-analytic approach to utilising public RNAseq data is extendable to include new
datasets and new species and provides a framework to support the Functional Annotation of Animals Genomes (FAANG) initiative.
FingerprintDive into the research topics of 'Functional annotation of the transcriptome of the pig, Sus scrofa, based upon network analysis of an RNAseq transcriptional atlas'. Together they form a unique fingerprint.
- 3 Finished
1/08/15 → 31/07/19
Tenesa, A., Archibald, A., Beard, P., Bishop, S., Bronsvoort, M., Burt, D., Freeman, T., Haley, C., Hocking, P., Houston, R., Hume, D., Joshi, A., Law, A., Michoel, T., Summers, K., Vernimmen, D., Watson, M., Wiener, P., Wilson, A., Woolliams, J., Ait-Ali, T., Barnett, M., Carlisle, A., Finlayson, H., Haga, I., Karavolos, M., Matika, O., Paterson, T., Paton, B., Pong-Wong, R., Robert, C. & Robertson, G.
1/04/12 → 31/03/17