Functional involvement of PHOSPHO1 in matrix vesicle-mediated skeletal mineralization

Scott Roberts; Sonoko Narisawa; Dympna Harmey; José Luis Millán; Colin Farquharson

doi:10.1359/jbmr.070108

Functional involvement of PHOSPHO1 in matrix vesicle-mediated skeletal mineralization

Scott Roberts, Sonoko Narisawa, Dympna Harmey, José Luis Millán, Colin Farquharson

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

PHOSPHO1 is a phosphatase highly expressed in bone. We studied its functional involvement in mineralization through the use of novel small molecule inhibitors. PHOSPHO1 expression was present within matrix vesicles, and inhibition of enzyme action caused a decrease in the ability of matrix vesicles to calcify.

Original language	English
Pages (from-to)	617-27
Number of pages	11
Journal	Journal of Bone and Mineral Research
Volume	22
Issue number	4
DOIs	https://doi.org/10.1359/jbmr.070108
Publication status	Published - Apr 2007

Keywords

2-Pyridinylmethylsulfinylbenzimidazoles
Alkaline Phosphatase
Animals
Base Sequence
Bone Matrix
Calcification, Physiologic
Cells, Cultured
Chickens
DNA Primers
Enzyme Inhibitors
Growth Plate
Humans
Immunohistochemistry
Male
Mice
Mice, Knockout
Osteoblasts
Phosphoric Monoester Hydrolases
Polymerase Chain Reaction
Thiadiazoles
Thiazoles

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Functional Involvement of PHOSPHO1 in Matrix Vesicle Mediated Skeletal MineralizationAccepted author manuscript, 266 KB

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title = "Functional involvement of PHOSPHO1 in matrix vesicle-mediated skeletal mineralization",

abstract = "PHOSPHO1 is a phosphatase highly expressed in bone. We studied its functional involvement in mineralization through the use of novel small molecule inhibitors. PHOSPHO1 expression was present within matrix vesicles, and inhibition of enzyme action caused a decrease in the ability of matrix vesicles to calcify.",

keywords = "2-Pyridinylmethylsulfinylbenzimidazoles, Alkaline Phosphatase, Animals, Base Sequence, Bone Matrix, Calcification, Physiologic, Cells, Cultured, Chickens, DNA Primers, Enzyme Inhibitors, Growth Plate, Humans, Immunohistochemistry, Male, Mice, Mice, Knockout, Osteoblasts, Phosphoric Monoester Hydrolases, Polymerase Chain Reaction, Thiadiazoles, Thiazoles",

author = "Scott Roberts and Sonoko Narisawa and Dympna Harmey and Mill{\'a}n, {Jos{\'e} Luis} and Colin Farquharson",

year = "2007",

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doi = "10.1359/jbmr.070108",

language = "English",

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AU - Roberts, Scott

AU - Narisawa, Sonoko

AU - Harmey, Dympna

AU - Millán, José Luis

AU - Farquharson, Colin

PY - 2007/4

Y1 - 2007/4

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AB - PHOSPHO1 is a phosphatase highly expressed in bone. We studied its functional involvement in mineralization through the use of novel small molecule inhibitors. PHOSPHO1 expression was present within matrix vesicles, and inhibition of enzyme action caused a decrease in the ability of matrix vesicles to calcify.

KW - 2-Pyridinylmethylsulfinylbenzimidazoles

KW - Alkaline Phosphatase

KW - Animals

KW - Base Sequence

KW - Bone Matrix

KW - Calcification, Physiologic

KW - Cells, Cultured

KW - Chickens

KW - DNA Primers

KW - Enzyme Inhibitors

KW - Growth Plate

KW - Humans

KW - Immunohistochemistry

KW - Male

KW - Mice

KW - Mice, Knockout

KW - Osteoblasts

KW - Phosphoric Monoester Hydrolases

KW - Polymerase Chain Reaction

KW - Thiadiazoles

KW - Thiazoles

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DO - 10.1359/jbmr.070108

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VL - 22

SP - 617

EP - 627

JO - Journal of Bone and Mineral Research

JF - Journal of Bone and Mineral Research

SN - 0884-0431

IS - 4

ER -

Functional involvement of PHOSPHO1 in matrix vesicle-mediated skeletal mineralization

Abstract

Keywords

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