Functional link between ribosome formation and biogenesis of iron-sulfur proteins

A  Yarunin; V G  Panse; E  Petfalski; C  Dez; D  Tollervey; E C  Hurt

doi:10.1038/sj.emboj.7600540

Functional link between ribosome formation and biogenesis of iron-sulfur proteins

A Yarunin, V G Panse, E Petfalski, C Dez, D Tollervey, E C Hurt

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in extramitochondrial iron - sulfur ( Fe - S) cluster biosynthesis. In particular, Nar1 was implicated to generate the Fe - S clusters within Rli1, a potential substrate protein of unknown function. We tested whether the Fe - S protein Rli1 functions in ribosome formation. We report that rli1 mutants are impaired in prerRNA processing and defective in the export of both ribosomal subunits. In addition, Rli1p is associated with both pre-40S particles and mature 40S subunits, and with the eIF3 translation initiation factor complex. Our data reveal an unexpected link between ribosome biogenesis and the biosynthetic pathway of cytoplasmic Fe - S proteins.

Original language	English
Pages (from-to)	580-588
Number of pages	9
Journal	EMBO Journal
Volume	24
Issue number	3
DOIs	https://doi.org/10.1038/sj.emboj.7600540
Publication status	Published - 9 Feb 2005

Keywords / Materials (for Non-textual outputs)

eIF3
iron–sulfur cluster
Nar1
ribosome export
Rli1

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10.1038/sj.emboj.7600540

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title = "Functional link between ribosome formation and biogenesis of iron-sulfur proteins",

abstract = "In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in extramitochondrial iron - sulfur ( Fe - S) cluster biosynthesis. In particular, Nar1 was implicated to generate the Fe - S clusters within Rli1, a potential substrate protein of unknown function. We tested whether the Fe - S protein Rli1 functions in ribosome formation. We report that rli1 mutants are impaired in prerRNA processing and defective in the export of both ribosomal subunits. In addition, Rli1p is associated with both pre-40S particles and mature 40S subunits, and with the eIF3 translation initiation factor complex. Our data reveal an unexpected link between ribosome biogenesis and the biosynthetic pathway of cytoplasmic Fe - S proteins.",

keywords = "eIF3, iron–sulfur cluster, Nar1, ribosome export, Rli1",

author = "A Yarunin and Panse, {V G} and E Petfalski and C Dez and D Tollervey and Hurt, {E C}",

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doi = "10.1038/sj.emboj.7600540",

language = "English",

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TY - JOUR

T1 - Functional link between ribosome formation and biogenesis of iron-sulfur proteins

AU - Yarunin, A

AU - Panse, V G

AU - Petfalski, E

AU - Dez, C

AU - Tollervey, D

AU - Hurt, E C

PY - 2005/2/9

Y1 - 2005/2/9

N2 - In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in extramitochondrial iron - sulfur ( Fe - S) cluster biosynthesis. In particular, Nar1 was implicated to generate the Fe - S clusters within Rli1, a potential substrate protein of unknown function. We tested whether the Fe - S protein Rli1 functions in ribosome formation. We report that rli1 mutants are impaired in prerRNA processing and defective in the export of both ribosomal subunits. In addition, Rli1p is associated with both pre-40S particles and mature 40S subunits, and with the eIF3 translation initiation factor complex. Our data reveal an unexpected link between ribosome biogenesis and the biosynthetic pathway of cytoplasmic Fe - S proteins.

AB - In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in extramitochondrial iron - sulfur ( Fe - S) cluster biosynthesis. In particular, Nar1 was implicated to generate the Fe - S clusters within Rli1, a potential substrate protein of unknown function. We tested whether the Fe - S protein Rli1 functions in ribosome formation. We report that rli1 mutants are impaired in prerRNA processing and defective in the export of both ribosomal subunits. In addition, Rli1p is associated with both pre-40S particles and mature 40S subunits, and with the eIF3 translation initiation factor complex. Our data reveal an unexpected link between ribosome biogenesis and the biosynthetic pathway of cytoplasmic Fe - S proteins.

KW - eIF3

KW - iron–sulfur cluster

KW - Nar1

KW - ribosome export

KW - Rli1

U2 - 10.1038/sj.emboj.7600540

DO - 10.1038/sj.emboj.7600540

M3 - Article

SN - 0261-4189

VL - 24

SP - 580

EP - 588

JO - EMBO Journal

JF - EMBO Journal

IS - 3

ER -

Functional link between ribosome formation and biogenesis of iron-sulfur proteins

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Keywords / Materials (for Non-textual outputs)

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