Functional properties of in vitro excitatory cortical neurons derived from human pluripotent stem cells

Matthew R Livesey, Dario Magnani, Giles E Hardingham, Siddharthan Chandran, David J A Wyllie

Research output: Contribution to journalArticlepeer-review

Abstract

The in vitro derivation of regionally defined human neuron types from patient-derived stem cells is now established as a resource to investigate human development and disease. Characterisation of such neurons initially focused on the expression of developmentally regulated transcription factors and neural markers, in conjunction with the development of protocols to direct and chart the fate of differentiated neurons. However, crucial to the understanding and exploitation of this technology is to determine the degree to which neurons recapitulate the key functional features exhibited by their native counterparts, essential for determining their usefulness in modelling human physiology and disease in vitro. Here, we review the emerging data concerning functional properties of human pluripotent stem cell-derived excitatory cortical neurons, both in the context of maturation and regional specificity. This article is protected by copyright. All rights reserved.

Original languageEnglish
JournalJournal of Physiology
Early online date26 Nov 2015
DOIs
Publication statusE-pub ahead of print - 26 Nov 2015

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