Functionally distinct subgroups of oligodendrocyte precursor cells integrate neural activity and execute myelin formation

Roberta Marisca, Tobias Hoche, Eneritz Agirre, Laura Jane Hoodless, Wenke Barkey, Franziska Auer, Gonçalo Castelo-Branco, Tim Czopka

Research output: Contribution to journalArticlepeer-review

Abstract

Recent reports have revealed that oligodendrocyte precursor cells (OPCs) are heterogeneous. It remains unclear whether such heterogeneity reflects different subtypes of cells with distinct functions or instead reflects transiently acquired states of cells with the same function. By integrating lineage formation of individual OPC clones, single-cell transcriptomics, calcium imaging and neural activity manipulation, we show that OPCs in the zebrafish spinal cord can be divided into two functionally distinct groups. One subgroup forms elaborate networks of processes and exhibits a high degree of calcium signaling, but infrequently differentiates despite contact with permissive axons. Instead, these OPCs divide in an activity- and calcium-dependent manner to produce another subgroup, with higher process motility and less calcium signaling and that readily differentiates. Our data show that OPC subgroups are functionally diverse in their response to neurons and that activity regulates the proliferation of a subset of OPCs that is distinct from the cells that generate differentiated oligodendrocytes.

Original languageEnglish
Pages (from-to)363-374
Number of pages12
JournalNature Neuroscience
Volume23
Issue number3
Early online date17 Feb 2020
DOIs
Publication statusPublished - 31 Mar 2020

Keywords

  • Animals
  • Animals, Genetically Modified
  • Calcium Signaling/physiology
  • Cell Differentiation
  • Cell Division
  • Cell Lineage
  • Cell Proliferation
  • Embryo, Nonmammalian/physiology
  • Myelin Sheath/physiology
  • Nerve Net/cytology
  • Oligodendrocyte Precursor Cells/physiology
  • Spinal Cord/cytology
  • Swimming/physiology
  • Zebrafish

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