Further studies on bovine serum amylase--the effect of gel buffer pH

A L Archibald, R L Spooner

Research output: Contribution to journalArticlepeer-review

Abstract

This paper describes the effect of gel buffer pH on the resolution of bovine serum amylase (Amylase I) isozymes in starch gel and the consequences for the understanding of the genetics of this locus. The two main findings are: (1) the existence of a satellite isozyme E to isozyme C which at pH 7.3 has the same mobility as the B isozyme but which at pH 8.0 migrates slower than B, and (2) the finding of three alleles Aml A, Aml B and Aml C in British cattle populations previously reported as having only Aml B and Aml C.
Original languageEnglish
Pages (from-to)229-38
Number of pages10
JournalAnimal blood groups and biochemical genetics
Volume9
Issue number4
Publication statusPublished - 1978

Keywords

  • Alleles
  • Amylases
  • Animals
  • Buffers
  • Cattle
  • Electrophoresis, Starch Gel
  • Hydrogen-Ion Concentration

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