Fusion of restriction termini using synthetic adaptor oligonucleotides

Richard Lathe*, Alain Balland, Vipin Kohli, Jean Pierre Lecocq

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

A method is proposed for linking a DNA fragment possessing a 5′ single-stranded extension to one carrying a 3′ extension. Synthetic oligonucleotide adaptors can be used to (i) change the site specificity at the termini of a fragment generated by restriction enzyme cleavage and (ii) simultaneously dephosphorylate the extremities of a DNA molecule to prevent recircularisation and allow positive selection for recombinant DNA molecules.

Original languageEnglish
Pages (from-to)187-195
Number of pages9
JournalGene
Volume20
Issue number2
DOIs
Publication statusPublished - 1982

Keywords / Materials (for Non-textual outputs)

  • dephosphorylation
  • ligation
  • Plasmid
  • recombinant DNA

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