Fusion of restriction termini using synthetic adaptor oligonucleotides

Richard Lathe; Alain Balland; Vipin Kohli; Jean Pierre Lecocq

doi:10.1016/0378-1119(82)90037-3

Fusion of restriction termini using synthetic adaptor oligonucleotides

Richard Lathe^*, Alain Balland, Vipin Kohli, Jean Pierre Lecocq

^*Corresponding author for this work

Deanery of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A method is proposed for linking a DNA fragment possessing a 5′ single-stranded extension to one carrying a 3′ extension. Synthetic oligonucleotide adaptors can be used to (i) change the site specificity at the termini of a fragment generated by restriction enzyme cleavage and (ii) simultaneously dephosphorylate the extremities of a DNA molecule to prevent recircularisation and allow positive selection for recombinant DNA molecules.

Original language	English
Pages (from-to)	187-195
Number of pages	9
Journal	Gene
Volume	20
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(82)90037-3
Publication status	Published - 1982

Keywords

dephosphorylation
ligation
Plasmid
recombinant DNA

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10.1016/0378-1119(82)90037-3

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abstract = "A method is proposed for linking a DNA fragment possessing a 5′ single-stranded extension to one carrying a 3′ extension. Synthetic oligonucleotide adaptors can be used to (i) change the site specificity at the termini of a fragment generated by restriction enzyme cleavage and (ii) simultaneously dephosphorylate the extremities of a DNA molecule to prevent recircularisation and allow positive selection for recombinant DNA molecules.",

keywords = "dephosphorylation, ligation, Plasmid, recombinant DNA",

author = "Richard Lathe and Alain Balland and Vipin Kohli and Lecocq, {Jean Pierre}",

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AU - Lathe, Richard

AU - Balland, Alain

AU - Kohli, Vipin

AU - Lecocq, Jean Pierre

PY - 1982

Y1 - 1982

N2 - A method is proposed for linking a DNA fragment possessing a 5′ single-stranded extension to one carrying a 3′ extension. Synthetic oligonucleotide adaptors can be used to (i) change the site specificity at the termini of a fragment generated by restriction enzyme cleavage and (ii) simultaneously dephosphorylate the extremities of a DNA molecule to prevent recircularisation and allow positive selection for recombinant DNA molecules.

AB - A method is proposed for linking a DNA fragment possessing a 5′ single-stranded extension to one carrying a 3′ extension. Synthetic oligonucleotide adaptors can be used to (i) change the site specificity at the termini of a fragment generated by restriction enzyme cleavage and (ii) simultaneously dephosphorylate the extremities of a DNA molecule to prevent recircularisation and allow positive selection for recombinant DNA molecules.

KW - dephosphorylation

KW - ligation

KW - Plasmid

KW - recombinant DNA

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DO - 10.1016/0378-1119(82)90037-3

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VL - 20

SP - 187

EP - 195

JO - Gene

JF - Gene

SN - 0378-1119

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ER -

Fusion of restriction termini using synthetic adaptor oligonucleotides

Abstract

Keywords

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