G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

Iain C Macaulay, Wilfried Haerty, Parveen Kumar, Yang I Li, Tim Xiaoming Hu, Mabel J Teng, Mubeen Goolam, Nathalie Saurat, Paul Coupland, Lesley M Shirley, Miriam Smith, Niels Van der Aa, Ruby Banerjee, Peter D Ellis, Michael A Quail, Harold P Swerdlow, Magdalena Zernicka-Goetz, Frederick J Livesey, Chris P Ponting, Thierry Voet

Research output: Contribution to journalArticlepeer-review


The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

Original languageEnglish
Pages (from-to)519-22
Number of pages4
JournalNature Methods
Issue number6
Publication statusPublished - 27 Mar 2015


  • Animals
  • Cell Line, Tumor
  • DNA
  • Genomics
  • Humans
  • Mice
  • Nucleic Acid Amplification Techniques
  • RNA, Messenger


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