Gas chromatography tandem mass spectrometry offers advantages for urinary steroids analysis

Natalie Homer, Sanjay Kothiya, Alison Rutter, Brian Walker, Ruth Andrew

Research output: Contribution to journalArticlepeer-review

Abstract

Gas chromatography mass spectrometry has been the lynchpin of clinical assessment of steroid profiles for ~3 decades. The improvements in assay performance offered by tandem mass spectrometry were assessed. Across the spectrum of glucocorticoid and androgen analytes tested, limits of detection and quantitation were ~20 fold lower with triple than single quadrupole systems, but the more noticeable improvement was that signal to noise was substantially improved and the linear range wider. These benefits allowed more reliable and concomitant measurement of steroids with substantially different abundances and in smaller
volumes of urine.
Original languageEnglish
JournalAnalytical Biochemistry
Volume538
Early online date6 Sep 2017
DOIs
Publication statusPublished - Dec 2017

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