Gel-based methods for the investigation of signal transduction pathways in Trypanosoma brucei

Balázs Szöőr, Mathieu Cayla

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract / Description of output

In the cell, reversible phosphorylation, controlled by protein phosphatases and protein kinases, initiates and regulates various signaling-dependent processes such as enzyme–substrate interactions, the cell cycle, differentiation, and immune responses. In addition to these processes, in unicellular parasites like Trypanosoma brucei, the causative agent of African sleeping sickness, additional signaling pathways have evolved to enable the survival of parasites in the changing environment of the vector and mammalian host. In this chapter, we describe two in vitro kinase assays and the use of the phosphoprotein chelator Phos-tag and show that these three polyacrylamide gel-based assays can be used for rapid target validation and detection of changes in phosphorylation.
Original languageEnglish
Title of host publicationTrypanosomatids
Subtitle of host publicationMethods and Protocols
ISBN (Electronic)978-1-0716-0294-2
ISBN (Print)978-1-0716-0293-5
Publication statusPublished - 28 Mar 2020

Publication series

ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords / Materials (for Non-textual outputs)

  • signal transduction
  • protein kinases
  • protein phosphatases
  • phospho substrate
  • Kinase assays
  • Phos-tag


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