Gene delivery of the elastase inhibitor elafin protects macrophages from neutrophil elastase-mediated impairment of apoptotic cell recognition

Peter A Henriksen, Andrew Devitt, Yuri Kotelevtsev, Jean-Michel Sallenave

Research output: Contribution to journalArticlepeer-review

Abstract

The resolution of inflammation is dependent on recognition and phagocytic removal of apoptotic cells by macrophages. Receptors for apoptotic cells are sensitive to degradation by human neutrophil elastase (HNE). We show in the present study that HNE cleaves macrophage cell surface CD14 and in so doing, reduces phagocytic recognition of apoptotic lymphocytic cells (Mutu 1). Using an improved method of adenovirus-mediated transfection of macrophages with the HNE inhibitor elafin, we demonstrate that elafin overexpression prevents CD14 cleavage and restores apoptotic cell recognition by macrophages. This approach of genetic modification of macrophages could be used to restore apoptotic cell recognition in inflammatory conditions.

Original languageEnglish
Pages (from-to)80-4
Number of pages5
JournalFEBS Letters
Volume574
Issue number1-3
DOIs
Publication statusPublished - 10 Sep 2004

Keywords

  • Apoptosis
  • Genetic Vectors
  • Humans
  • Leukocyte Elastase/antagonists & inhibitors
  • Macrophages/drug effects
  • Proteinase Inhibitory Proteins, Secretory
  • Proteins/administration & dosage

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