Gene expression patterns observed from the skin of selected cattle breeds infested with Amblyomma hebraeum ticks

M Letuka, D. Wragg, A Bester-van der Merwe, Kennedy Dzama, LT Nesengani, Oswald Matika, B Dube, N. O. Mapholi

Research output: Contribution to conferencePaperpeer-review

Abstract / Description of output

This study aimed to identify genes that mediate immune responses in tick-tolerant Nguni, tick-susceptible Angus, and F2 Nguni X Angus population, infested with Amblyomma hebraeum ticks. Skin biopsy samples were collected prior and at post-infestation. RNA was isolated from the skin biopsies and sequenced on the Illumina HiSeq2500 Illumina sequencer. Overall, 139 significantly differentially expressed genes were identified in the current study. Calcium signalling pathway was the most enriched pathway linked to Five genes (PHKG1, PHKA1, CAMK21, NOS2, HRC). NOS2 gene was highly (significantly, p>0.05) expressed in the susceptible group before tick infestation and highly expressed in resistant group after tick infestation. Similar pattern was observed with HRC gene. HRC gene has been previously reported to play a role in regulating immune response against infections. This research reports differential gene expression following tick bites, thus providing preliminary data concerning the underlying mechanisms associated with the immune response.
Original languageEnglish
Publication statusE-pub ahead of print - 3 Jul 2022
EventWorld Congress on Genetics Applied to Livestock - Rotterdam, Netherlands
Duration: 3 Jul 20228 Jul 2022
https://wcgalp.com/scientific-programme

Conference

ConferenceWorld Congress on Genetics Applied to Livestock
Abbreviated titleWCGALP
Country/TerritoryNetherlands
Period3/07/228/07/22
Internet address

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