Gene expression profiling of primary canine insulinomas and their metastases

Floryne Buishand, Jolle Kirpensteijn, Alexandra A Jaarsma, Ernst-Jan M Speel, Marja Kik, Jan A Mol

Research output: Contribution to journalArticlepeer-review

Abstract

The gene expression profile of 10 primary canine insulinomas was compared with that of their accompanying metastases using microarray analysis and quantitative real time-PCR. Analysis of microarray data revealed 84 genes that were differentially expressed between primary insulinomas and their metastases, along with 243 genes differentially expressed between a low-metastatic and a high-metastatic subset of primary insulinomas. The genes differently expressed between primary insulinomas and their metastases clustered together in nine signalling pathways. Comparing the low-metastatic to the high-metastatic subset of primary insulinomas, 26 pathways appeared to be significantly influenced. The acinar enzymes pancreatic lipase (PNLIP) and chymotrypsinogen B1 (CTRB1) were amongst the most down-regulated genes in the malignant group of primary insulinomas and in metastases. Immunofluorescence demonstrated co-localisation of insulin and PNLIP in tumour cells. Different subsets of canine insulinomas can be identified on the basis of their gene expression profile. Canine insulinomas appear to contain amphicrine cells, which exhibit both endocrine and exocrine cell features.

Original languageEnglish
Pages (from-to)192-7
Number of pages6
JournalVeterinary Journal
Volume197
Issue number2
Early online date18 Feb 2013
DOIs
Publication statusE-pub ahead of print - 18 Feb 2013

Keywords

  • Animals
  • Dog Diseases
  • Dogs
  • Female
  • Gene Expression Regulation, Neoplastic
  • Insulinoma
  • Male
  • Neoplasm Metastasis
  • Soft Tissue Neoplasms
  • Transcriptome
  • Journal Article

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