Gene targeting in primary fetal fibroblasts from sheep and pig

C Denning; P Dickinson; S Burl; D Wylie; J Fletcher; A J Clark

doi:10.1089/15362300152725945

Gene targeting in primary fetal fibroblasts from sheep and pig

C Denning, P Dickinson, S Burl, D Wylie, J Fletcher, A J Clark

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Nuclear transfer offers a new cell-based route for introducing precise genetic modifications in a range of animal species. However, significant challenges, such as establishment of somatic gene targeting techniques, must be overcome before the technology can be applied routinely. In this report, we describe targeted deletion at the GGTA1 (alpha 1,3-galactosyl transferase) and PrP (prion protein) loci in primary fibroblasts from livestock. We place particular emphasis on the growth characteristics of the primary cell cultures, since these are key to determining success.

Original language	English
Pages (from-to)	221-31
Number of pages	11
Journal	Cloning and Stem Cells
Volume	3
Issue number	4
DOIs	https://doi.org/10.1089/15362300152725945
Publication status	Published - 2001

Keywords / Materials (for Non-textual outputs)

Animals
Animals, Newborn
Blotting, Northern
Cell Nucleus
DNA Primers
Fetus
Fibroblasts
Galactosyltransferases
Gene Deletion
Gene Targeting
Gene Transfer Techniques
Genetic Vectors
Karyotyping
Models, Genetic
Nuclear Transfer Techniques
Polymerase Chain Reaction
Prions
Sheep
Swine

Access to Document

10.1089/15362300152725945

Denning et al 2001Accepted author manuscript, 308 KB

http://online.liebertpub.com/doi/abs/10.1089/15362300152725945

Cite this

@article{33e47bb6663644d2bd14679d0fcb20ad,

title = "Gene targeting in primary fetal fibroblasts from sheep and pig",

abstract = "Nuclear transfer offers a new cell-based route for introducing precise genetic modifications in a range of animal species. However, significant challenges, such as establishment of somatic gene targeting techniques, must be overcome before the technology can be applied routinely. In this report, we describe targeted deletion at the GGTA1 (alpha 1,3-galactosyl transferase) and PrP (prion protein) loci in primary fibroblasts from livestock. We place particular emphasis on the growth characteristics of the primary cell cultures, since these are key to determining success.",

keywords = "Animals, Animals, Newborn, Blotting, Northern, Cell Nucleus, DNA Primers, Fetus, Fibroblasts, Galactosyltransferases, Gene Deletion, Gene Targeting, Gene Transfer Techniques, Genetic Vectors, Karyotyping, Models, Genetic, Nuclear Transfer Techniques, Polymerase Chain Reaction, Prions, Sheep, Swine",

author = "C Denning and P Dickinson and S Burl and D Wylie and J Fletcher and Clark, {A J}",

year = "2001",

doi = "10.1089/15362300152725945",

language = "English",

volume = "3",

pages = "221--31",

journal = "Cloning and Stem Cells",

issn = "1536-2302",

publisher = "Mary Ann Liebert Inc.",

number = "4",

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TY - JOUR

T1 - Gene targeting in primary fetal fibroblasts from sheep and pig

AU - Denning, C

AU - Dickinson, P

AU - Burl, S

AU - Wylie, D

AU - Fletcher, J

AU - Clark, A J

PY - 2001

Y1 - 2001

N2 - Nuclear transfer offers a new cell-based route for introducing precise genetic modifications in a range of animal species. However, significant challenges, such as establishment of somatic gene targeting techniques, must be overcome before the technology can be applied routinely. In this report, we describe targeted deletion at the GGTA1 (alpha 1,3-galactosyl transferase) and PrP (prion protein) loci in primary fibroblasts from livestock. We place particular emphasis on the growth characteristics of the primary cell cultures, since these are key to determining success.

AB - Nuclear transfer offers a new cell-based route for introducing precise genetic modifications in a range of animal species. However, significant challenges, such as establishment of somatic gene targeting techniques, must be overcome before the technology can be applied routinely. In this report, we describe targeted deletion at the GGTA1 (alpha 1,3-galactosyl transferase) and PrP (prion protein) loci in primary fibroblasts from livestock. We place particular emphasis on the growth characteristics of the primary cell cultures, since these are key to determining success.

KW - Animals

KW - Animals, Newborn

KW - Blotting, Northern

KW - Cell Nucleus

KW - DNA Primers

KW - Fetus

KW - Fibroblasts

KW - Galactosyltransferases

KW - Gene Deletion

KW - Gene Targeting

KW - Gene Transfer Techniques

KW - Genetic Vectors

KW - Karyotyping

KW - Models, Genetic

KW - Nuclear Transfer Techniques

KW - Polymerase Chain Reaction

KW - Prions

KW - Sheep

KW - Swine

U2 - 10.1089/15362300152725945

DO - 10.1089/15362300152725945

M3 - Article

C2 - 11945232

SN - 1536-2302

VL - 3

SP - 221

EP - 231

JO - Cloning and Stem Cells

JF - Cloning and Stem Cells

IS - 4

ER -

Gene targeting in primary fetal fibroblasts from sheep and pig

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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