Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies

Aina Segués, Sander M.j. Van Duijnhoven, Marc Parade, Lilian Driessen, Nataša Vukovic, Dietmar Zaiss, Alice J.A.M. Sijts, Pedro Berraondo, Andrea Van Elsas

Research output: Contribution to journalArticlepeer-review

Abstract

Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8+ T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8+ cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer.

Original languageEnglish
Article number113173
JournalJournal of Immunological Methods
Volume499
Early online date24 Oct 2021
DOIs
Publication statusPublished - 1 Dec 2021

Keywords

  • TNFR2
  • antibody
  • epitope
  • cysteine-rich domain
  • costimulation
  • treg

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